Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 81621..82198 Replicon plasmid unnamed2
Accession NZ_CP041094
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPCTRSRTH01

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag CI667_RS27125 Protein ID WP_059065587.1
Coordinates 81621..81953 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag CI667_RS27130 Protein ID WP_032454911.1
Coordinates 81953..82198 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CI667_RS27090 77719..77949 + 231 WP_004118481.1 hypothetical protein -
CI667_RS27100 78472..78774 + 303 Protein_75 antirestriction protein -
CI667_RS27105 78728..78988 + 261 Protein_76 hypothetical protein -
CI667_RS27110 79223..80626 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
CI667_RS27115 80655..81287 - 633 WP_001567369.1 hypothetical protein -
CI667_RS27120 81464..81556 - 93 Protein_79 IS3 family transposase -
CI667_RS27125 81621..81953 - 333 WP_059065587.1 endoribonuclease MazF Toxin
CI667_RS27130 81953..82198 - 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CI667_RS27135 82621..82701 + 81 Protein_82 IS1 family transposase -
CI667_RS27140 82836..83375 + 540 WP_094308945.1 hypothetical protein -
CI667_RS27145 83372..84181 + 810 WP_094308943.1 ATP-binding cassette domain-containing protein -
CI667_RS27150 84614..84802 - 189 Protein_85 transposase -
CI667_RS27155 84807..85976 + 1170 Protein_86 UxaA family hydrolase -
CI667_RS27160 86045..86854 - 810 WP_015369557.1 DeoR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD / iucC / iucB / iucA / cseA 1..168755 168755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T127592 WP_059065587.1 NZ_CP041094:c81953-81621 [Klebsiella pneumoniae subsp. pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T127592 NZ_CP041094:c81953-81621 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGCGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT127592 WP_032454911.1 NZ_CP041094:c82198-81953 [Klebsiella pneumoniae subsp. pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT127592 NZ_CP041094:c82198-81953 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCGGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References