Detailed information of TA system TA127584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3953371..3953990
• Replicon: chromosome
• Accession: NZ_CP041092
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPCTRSRTH01

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CI667_RS19295
• Protein ID: WP_002892050.1
• Coordinates: 3953772..3953990 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CI667_RS19290
• Protein ID: WP_094488106.1
• Coordinates: 3953371..3953745 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI667_RS19280	3948523..3949716	+	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CI667_RS19285	3949739..3952885	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
CI667_RS19290	3953371..3953745	+	375	WP_094488106.1	Hha toxicity modulator TomB	Antitoxin
CI667_RS19295	3953772..3953990	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
CI667_RS19300	3954149..3954715	+	567	WP_002892030.1	maltose O-acetyltransferase	-
CI667_RS28700	3954687..3954827	-	141	WP_004147370.1	hypothetical protein	-
CI667_RS19305	3954848..3955318	+	471	WP_002892026.1	YlaC family protein	-
CI667_RS19310	3955293..3956744	-	1452	WP_094488105.1	PLP-dependent aminotransferase family protein	-
CI667_RS19315	3956845..3957543	+	699	WP_002892021.1	GNAT family N-acetyltransferase	-
CI667_RS19320	3957540..3957680	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
CI667_RS19325	3957680..3957943	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T127584 WP_002892050.1 NZ_CP041092:3953772-3953990 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T127584 NZ_CP041092:3953772-3953990 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14338.03 Da
• Isoelectric Point: 4.8989

>AT127584 WP_094488106.1 NZ_CP041092:3953371-3953745 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT127584 NZ_CP041092:3953371-3953745 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCGCATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2