Detailed information of TA system TA127574

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1169474..1170207
• Replicon: chromosome
• Accession: NZ_CP041092
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPCTRSRTH01

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: CI667_RS05830
• Protein ID: WP_094487661.1
• Coordinates: 1169474..1169803 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CI667_RS05835
• Protein ID: WP_094487662.1
• Coordinates: 1169842..1170207 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI667_RS05805	1164527..1165702	-	1176	WP_002914189.1	multidrug efflux RND transporter periplasmic adaptor subunit OqxA	-
CI667_RS05810	1166036..1166398	+	363	WP_002914181.1	helix-turn-helix domain-containing protein	-
CI667_RS05815	1166409..1166981	-	573	WP_087829996.1	flavin reductase	-
CI667_RS05820	1167193..1168077	+	885	WP_002914178.1	LysR family transcriptional regulator	-
CI667_RS05825	1168202..1169002	+	801	WP_062913715.1	hypothetical protein	-
CI667_RS05830	1169474..1169803	-	330	WP_094487661.1	TA system toxin CbtA family protein	Toxin
CI667_RS05835	1169842..1170207	-	366	WP_094487662.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CI667_RS05840	1170233..1170454	-	222	WP_094487663.1	DUF987 family protein	-
CI667_RS05845	1170451..1170993	-	543	WP_094487664.1	DNA repair protein RadC	-
CI667_RS05850	1171006..1171449	-	444	WP_094487665.1	antirestriction protein	-
CI667_RS05855	1171480..1172301	-	822	WP_094487666.1	DUF945 domain-containing protein	-
CI667_RS05860	1172393..1172734	-	342	WP_032616287.1	hypothetical protein	-
CI667_RS05865	1172769..1173233	-	465	WP_094487667.1	hypothetical protein	-
CI667_RS05875	1173359..1173517	-	159	WP_088776065.1	hypothetical protein	-
CI667_RS05880	1173730..1174617	-	888	WP_094487668.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12487.20 Da
• Isoelectric Point: 9.5061

>T127574 WP_094487661.1 NZ_CP041092:c1169803-1169474 [Klebsiella pneumoniae subsp. pneumoniae]
MQTLSSHPTRATQPYLSPVETWQRLLTHLLSQHYGLTLNDTPFSNETTIREHIDAGVSLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVMDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T127574 NZ_CP041092:c1169803-1169474 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAAACCTTATCATCACACCCGACACGGGCGACTCAGCCCTATCTGTCACCCGTTGAAACCTGGCAACGGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTAATGAAACCACCATACGAGAACATATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATACGGGCTTGTCCGTATCGATCGGAAAGGATTT
TCGTGGCAAGAGCAGACCCCGTATCTTTCCGTAATGGATATTTTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
CGTGAAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13514.25 Da
• Isoelectric Point: 5.4911

>AT127574 WP_094487662.1 NZ_CP041092:c1170207-1169842 [Klebsiella pneumoniae subsp. pneumoniae]
MNNHSESGTKPENLTCQQWGLKRTIIPCFGARLVQEGNRVHFLADRAGFNGAFSDDDALHLDQAFPLILKKLELMLTSGE
LNPRYQHCVTLYHNGLACEADTLGSCGYVYIAIYPDQTEPQ

Nucleotide

• Length: 366 bp

>AT127574 NZ_CP041092:c1170207-1169842 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAACAATCACTCAGAATCCGGTACCAAACCGGAGAATCTTACCTGCCAACAGTGGGGACTAAAGCGCACGATCATCCC
CTGTTTTGGCGCTCGTCTGGTGCAGGAAGGCAACCGAGTGCATTTTCTCGCTGACCGGGCCGGATTCAACGGTGCTTTCA
GCGACGATGACGCGCTACACCTGGATCAGGCCTTTCCCCTGATACTCAAAAAACTGGAACTAATGCTGACCAGCGGCGAG
CTCAATCCACGCTATCAGCACTGCGTCACGCTTTACCACAACGGGCTCGCTTGCGAAGCCGACACACTCGGTTCCTGTGG
CTATGTCTATATTGCTATTTATCCTGACCAGACTGAGCCGCAGTAA