Detailed information of TA system TA127568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4445747..4446287
• Replicon: chromosome
• Accession: NZ_CP041090
• Organism: Bradyrhizobium symbiodeficiens strain 65S1MB

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FJN17_RS21035
• Protein ID: WP_094977258.1
• Coordinates: 4446027..4446287 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FJN17_RS21030
• Protein ID: WP_094977259.1
• Coordinates: 4445747..4446040 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJN17_RS21005	4441579..4442430	-	852	WP_094977262.1	3-mercaptopyruvate sulfurtransferase	-
FJN17_RS21010	4442591..4443004	-	414	WP_094977261.1	rhodanese-like domain-containing protein	-
FJN17_RS21015	4443158..4444333	-	1176	WP_140480938.1	VWA domain-containing protein	-
FJN17_RS21020	4444456..4444701	+	246	WP_018318587.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
FJN17_RS21025	4444797..4445639	+	843	WP_027529387.1	MoxR family ATPase	-
FJN17_RS21030	4445747..4446040	-	294	WP_094977259.1	BrnA antitoxin family protein	Antitoxin
FJN17_RS21035	4446027..4446287	-	261	WP_094977258.1	BrnT family toxin	Toxin
FJN17_RS21040	4446471..4448117	-	1647	WP_140480939.1	GMC family oxidoreductase N-terminal domain-containing protein	-
FJN17_RS21045	4448135..4448728	-	594	WP_094893798.1	GNAT family N-acetyltransferase	-
FJN17_RS21050	4448888..4449814	-	927	WP_140480940.1	SMP-30/gluconolactonase/LRE family protein	-
FJN17_RS21055	4450063..4451166	+	1104	WP_140480941.1	mandelate racemase/muconate lactonizing enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9864.46 Da
• Isoelectric Point: 10.5508

>T127568 WP_094977258.1 NZ_CP041090:c4446287-4446027 [Bradyrhizobium symbiodeficiens]
MKIVWDEPKRLANLDKHELDFADLNETFFDNALVIPSHNKSKRWVAIGVSIRGVIVVVFARLGREGVSIISMRPASRSER
KLYAER

Nucleotide

• Length: 261 bp

>T127568 NZ_CP041090:c4446287-4446027 [Bradyrhizobium symbiodeficiens]
ATGAAAATCGTCTGGGATGAACCGAAGCGGCTCGCCAACCTCGACAAGCACGAGCTGGATTTTGCCGATCTCAACGAGAC
GTTCTTCGATAACGCACTGGTCATTCCGTCGCACAACAAGAGCAAACGCTGGGTTGCGATCGGCGTGAGCATCCGTGGTG
TCATTGTTGTCGTGTTCGCCCGGCTGGGACGCGAAGGCGTCAGCATCATCAGCATGCGACCGGCAAGCCGCAGCGAAAGG
AAACTCTATGCCGAACGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10779.42 Da
• Isoelectric Point: 11.3548

>AT127568 WP_094977259.1 NZ_CP041090:c4446040-4445747 [Bradyrhizobium symbiodeficiens]
MPNAKKKSGYTKRDLRAVSDTPELTRADFAKARPFSEVFPDLSAAIRKGRGPNKAPTKTLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT127568 NZ_CP041090:c4446040-4445747 [Bradyrhizobium symbiodeficiens]
ATGCCGAACGCTAAAAAGAAGTCGGGTTACACCAAGAGAGACCTGCGTGCGGTCAGCGACACTCCGGAGCTGACAAGGGC
CGATTTCGCCAAGGCGCGGCCTTTCTCTGAAGTCTTCCCTGATCTCAGCGCAGCCATCCGCAAGGGCCGAGGTCCGAACA
AGGCGCCGACAAAGACGCTCGTCTCCCTGCGCCTCAGCCCAGAGGTGGTCGAGCATTTCAAATCGACCGGACCGGGCTGG
CAGTCGCGGATCGACGAAACCTTGCGCAAGGCGGTGAAGCGCAAGGCGTCGTGA