Detailed information of TA system TA127565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 34611..35254
• Replicon: plasmid pKp202_4
• Accession: NZ_CP041086
• Organism: Klebsiella pneumoniae strain Kp202

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: FJV42_RS29670
• Protein ID: WP_001044770.1
• Coordinates: 34838..35254 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: FJV42_RS29665
• Protein ID: WP_001261282.1
• Coordinates: 34611..34841 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJV42_RS29635	30183..31049	-	867	WP_004118283.1	replication initiation protein	-
FJV42_RS30630	31584..31688	+	105	WP_032409716.1	hypothetical protein	-
FJV42_RS29640	31817..32074	+	258	WP_000764642.1	hypothetical protein	-
FJV42_RS29645	32132..32908	-	777	WP_000015958.1	site-specific integrase	-
FJV42_RS29650	32905..33648	-	744	WP_000129823.1	hypothetical protein	-
FJV42_RS29655	33699..34049	-	351	WP_000493378.1	hypothetical protein	-
FJV42_RS29660	34193..34654	-	462	WP_072202616.1	hypothetical protein	-
FJV42_RS29665	34611..34841	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FJV42_RS29670	34838..35254	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
FJV42_RS29675	35328..36038	+	711	WP_004152334.1	ATP-binding protein	-
FJV42_RS29680	36072..36769	+	698	Protein_48	IS1 family transposase	-
FJV42_RS29685	36770..36895	+	126	WP_072093211.1	mercury transporter	-
FJV42_RS29690	36931..37353	+	423	WP_001340589.1	organomercurial transporter MerC	-
FJV42_RS29695	37405..39099	+	1695	WP_000105636.1	mercury(II) reductase	-
FJV42_RS29700	39117..39479	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
FJV42_RS29705	39476..39712	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1A / blaCTX-M-15 / dfrA14	-	1..61718	61718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T127565 WP_001044770.1 NZ_CP041086:34838-35254 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T127565 NZ_CP041086:34838-35254 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT127565 WP_001261282.1 NZ_CP041086:34611-34841 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT127565 NZ_CP041086:34611-34841 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3