Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53664..54400 Replicon plasmid pKp202_2
Accession NZ_CP041084
Organism Klebsiella pneumoniae strain Kp202

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FJV42_RS28320 Protein ID WP_003026803.1
Coordinates 53918..54400 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FJV42_RS28315 Protein ID WP_003026799.1
Coordinates 53664..53930 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJV42_RS28270 49726..50088 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
FJV42_RS28275 50138..50488 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
FJV42_RS28280 50846..51115 + 270 WP_004152102.1 hypothetical protein -
FJV42_RS28285 51103..51678 + 576 WP_004152103.1 hypothetical protein -
FJV42_RS28290 51709..52203 + 495 WP_004152104.1 DNA-binding protein -
FJV42_RS28295 52247..52615 + 369 WP_004152105.1 hypothetical protein -
FJV42_RS28300 52649..52852 + 204 WP_004152106.1 hemolysin expression modulator Hha -
FJV42_RS28305 52901..53158 + 258 WP_004152107.1 hypothetical protein -
FJV42_RS28310 53234..53488 + 255 WP_004152108.1 hypothetical protein -
FJV42_RS28315 53664..53930 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FJV42_RS28320 53918..54400 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FJV42_RS28325 54601..56004 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
FJV42_RS28330 56033..56665 - 633 WP_001567369.1 hypothetical protein -
FJV42_RS28335 56895..58241 + 1347 WP_020314316.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSCO-1 / aac(3)-IIa / blaTEM-1B - 1..158439 158439


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T127564 WP_003026803.1 NZ_CP041084:53918-54400 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T127564 NZ_CP041084:53918-54400 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT127564 WP_003026799.1 NZ_CP041084:53664-53930 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT127564 NZ_CP041084:53664-53930 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References