Detailed information of TA system TA127544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 697892..698528
• Replicon: chromosome
• Accession: NZ_CP041066
• Organism: Priestia megaterium strain KNU-01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: FJM50_RS05085
• Protein ID: WP_013055004.1
• Coordinates: 698178..698528 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: FJM50_RS05080
• Protein ID: WP_013055003.1
• Coordinates: 697892..698173 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJM50_RS05055	693258..694229	+	972	WP_035439664.1	UV DNA damage repair endonuclease UvsE	-
FJM50_RS05060	694238..694840	-	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
FJM50_RS05065	694905..695270	+	366	WP_029325479.1	holo-ACP synthase	-
FJM50_RS05070	695329..696387	+	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
FJM50_RS05075	696501..697691	+	1191	WP_013055002.1	alanine racemase	-
FJM50_RS05080	697892..698173	+	282	WP_013055003.1	hypothetical protein	Antitoxin
FJM50_RS05085	698178..698528	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FJM50_RS05090	698686..699522	+	837	WP_013055005.1	STAS domain-containing protein	-
FJM50_RS05095	699525..699881	+	357	WP_013055006.1	STAS domain-containing protein	-
FJM50_RS05100	699885..700286	+	402	WP_013055007.1	anti-sigma regulatory factor	-
FJM50_RS05105	700300..701310	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
FJM50_RS05110	701370..701702	+	333	WP_013055009.1	anti-sigma factor antagonist	-
FJM50_RS05115	701699..702184	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
FJM50_RS05120	702150..702944	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T127544 WP_013055004.1 NZ_CP041066:698178-698528 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T127544 NZ_CP041066:698178-698528 [Priestia megaterium]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT127544 WP_013055003.1 NZ_CP041066:697892-698173 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT127544 NZ_CP041066:697892-698173 [Priestia megaterium]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9