Detailed information of TA system TA127543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 2903787..2904420
• Replicon: chromosome
• Accession: NZ_CP041063
• Organism: Bacillus sp. Cs-700

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A0J6CT91
• Locus tag: FJM75_RS14680
• Protein ID: WP_048312992.1
• Coordinates: 2904070..2904420 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FJM75_RS14675
• Protein ID: WP_169525642.1
• Coordinates: 2903787..2904065 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJM75_RS14655	2899059..2899418	+	360	WP_165999254.1	holo-ACP synthase	-
FJM75_RS14660	2899500..2901005	+	1506	WP_207393279.1	NAD(P)H-hydrate dehydratase	-
FJM75_RS14665	2901068..2902069	+	1002	WP_165999258.1	outer membrane lipoprotein carrier protein LolA	-
FJM75_RS14670	2902486..2903652	+	1167	WP_165999260.1	alanine racemase	-
FJM75_RS14675	2903787..2904065	+	279	WP_169525642.1	antitoxin	Antitoxin
FJM75_RS14680	2904070..2904420	+	351	WP_048312992.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FJM75_RS14685	2904698..2905519	+	822	WP_165999262.1	STAS domain-containing protein	-
FJM75_RS14690	2905532..2905888	+	357	WP_048312990.1	STAS domain-containing protein	-
FJM75_RS14695	2905892..2906293	+	402	WP_165999265.1	anti-sigma regulatory factor	-
FJM75_RS14700	2906310..2907320	+	1011	WP_160921736.1	PP2C family protein-serine/threonine phosphatase	-
FJM75_RS14705	2907396..2907725	+	330	WP_098446066.1	STAS domain-containing protein	-
FJM75_RS14710	2907749..2908204	+	456	WP_159786998.1	anti-sigma B factor RsbW	-
FJM75_RS14715	2908173..2908964	+	792	WP_098446067.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13002.09 Da
• Isoelectric Point: 6.4695

>T127543 WP_048312992.1 NZ_CP041063:2904070-2904420 [Bacillus sp. Cs-700]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEGMMQRVQEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T127543 NZ_CP041063:2904070-2904420 [Bacillus sp. Cs-700]
TTGATTGTCAAACGAGGTGATGTGTATTTTGCTGACCTTTCTCCTGTTGTAGGTTCTGAACAAGGTGGCGTAAGACCCGT
CTTGATTATTCAGAACGACATTGGGAATCGTTTTAGTCCTACTGTCATTGTTGCTGCAATCACTGCGCAAATTCAGAAAG
CAAAGCTTCCCACACATGTCGAAATCGATGCGAAGCGCTATGGATTTGAACGTGATTCGGTTATACTGCTAGAGCAAATC
CGTACTATTGATAAACAGCGTCTAACTGATAAAATCACTCACCTTGATGAGGGTATGATGCAAAGGGTACAAGAAGCTTT
GCAAATCAGCCTTGGACTTATTGATTTTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10600.10 Da
• Isoelectric Point: 5.1695

>AT127543 WP_169525642.1 NZ_CP041063:2903787-2904065 [Bacillus sp. Cs-700]
VSEANKKSIVVTLPQHILNEVDGIIQQEQLDRNEFISQATTMYIRERKKRQIRDAMRQGYMEMAKINLNLAAEAFLVEEE
AEHTVDRLVSGV

Nucleotide

• Length: 279 bp

>AT127543 NZ_CP041063:2903787-2904065 [Bacillus sp. Cs-700]
GTGTCAGAGGCAAACAAAAAATCGATTGTTGTAACTCTCCCACAACATATTTTAAATGAGGTGGATGGTATTATTCAACA
GGAACAGCTTGATCGTAACGAGTTCATATCACAGGCCACGACAATGTATATTCGCGAACGAAAGAAGCGACAAATTCGTG
ACGCCATGCGCCAGGGATACATGGAGATGGCTAAGATTAATTTGAACCTTGCAGCAGAAGCCTTTCTTGTTGAGGAGGAA
GCAGAGCACACCGTGGACCGCTTAGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0J6CT91