Detailed information of TA system TA127532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 483233..483704
• Replicon: chromosome
• Accession: NZ_CP041062
• Organism: Enterobacter ludwigii strain I140

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECI140_RS02320
• Protein ID: WP_138613169.1
• Coordinates: 483465..483704 (+)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ECI140_RS02315
• Protein ID: WP_047743215.1
• Coordinates: 483233..483475 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECI140_RS02300	479263..480003	+	741	WP_014168382.1	KDGP aldolase family protein	-
ECI140_RS02305	480089..481225	+	1137	WP_138613167.1	lactonase family protein	-
ECI140_RS02310	481245..483155	+	1911	WP_014168384.1	BglG family transcription antiterminator	-
ECI140_RS02315	483233..483475	+	243	WP_047743215.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ECI140_RS02320	483465..483704	+	240	WP_138613169.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECI140_RS02325	483753..484217	-	465	WP_032681844.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
ECI140_RS02330	484356..486494	-	2139	WP_020885401.1	anaerobic ribonucleoside-triphosphate reductase	-
ECI140_RS02335	486862..487434	-	573	WP_025205763.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-77)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9063.60 Da
• Isoelectric Point: 9.9348

>T127532 WP_138613169.1 NZ_CP041062:483465-483704 [Enterobacter ludwigii]
MTYELVFDPRAFKEWARLGDTVKNQFKKKLANVLVNPRVESARLHGLPDCDKIKLRSQGYRLVYQVQDNVVTVCVIAIG

Nucleotide

• Length: 240 bp

>T127532 NZ_CP041062:483465-483704 [Enterobacter ludwigii]
ATGACTTATGAACTCGTATTTGATCCGAGGGCGTTCAAGGAGTGGGCCAGGCTGGGTGATACCGTAAAAAATCAGTTTAA
GAAGAAGCTTGCGAACGTGTTGGTGAACCCCAGGGTTGAATCGGCTCGCCTGCATGGCCTGCCTGATTGCGACAAGATAA
AACTCAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAACGTTGTGACGGTATGTGTTATTGCTATCGGTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9071.46 Da
• Isoelectric Point: 5.1088

>AT127532 WP_047743215.1 NZ_CP041062:483233-483475 [Enterobacter ludwigii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDELIQTVRERLANPQKGVKVRLDDL

Nucleotide

• Length: 243 bp

>AT127532 NZ_CP041062:483233-483475 [Enterobacter ludwigii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACGCC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCGTATGCCGGTGAAAACGGTCACTGTCAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAGTTAAGGTTAGGCTGGATGACTTA
TGA