Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1288671..1289291 Replicon chromosome
Accession NZ_CP041051
Organism Citrobacter sp. CF971

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag E6P06_RS09630 Protein ID WP_002892050.1
Coordinates 1288671..1288889 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag E6P06_RS09635 Protein ID WP_003021733.1
Coordinates 1288917..1289291 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6P06_RS09595 1283904..1284533 + 630 WP_016149709.1 membrane protein -
E6P06_RS09600 1284598..1284951 + 354 WP_003831033.1 DUF1428 family protein -
E6P06_RS09605 1285003..1286556 - 1554 WP_046669815.1 EAL domain-containing protein -
E6P06_RS09610 1286745..1287005 + 261 WP_003835926.1 type B 50S ribosomal protein L31 -
E6P06_RS09615 1287007..1287147 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
E6P06_RS09620 1287226..1287696 - 471 WP_003021724.1 YlaC family protein -
E6P06_RS09625 1287813..1288364 - 552 WP_003835924.1 maltose O-acetyltransferase -
E6P06_RS09630 1288671..1288889 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
E6P06_RS09635 1288917..1289291 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
E6P06_RS09640 1289780..1292929 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
E6P06_RS09645 1292952..1294145 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T127517 WP_002892050.1 NZ_CP041051:c1288889-1288671 [Citrobacter sp. CF971]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T127517 NZ_CP041051:c1288889-1288671 [Citrobacter sp. CF971]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT127517 WP_003021733.1 NZ_CP041051:c1289291-1288917 [Citrobacter sp. CF971]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT127517 NZ_CP041051:c1289291-1288917 [Citrobacter sp. CF971]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References