Detailed information of TA system TA127514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 311657..312323
• Replicon: chromosome
• Accession: NZ_CP041051
• Organism: Citrobacter sp. CF971

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: E6P06_RS04945
• Protein ID: WP_003844680.1
• Coordinates: 311657..312016 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0D7LIR0
• Locus tag: E6P06_RS04950
• Protein ID: WP_003844682.1
• Coordinates: 312006..312323 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P06_RS04935	309057..310688	+	1632	WP_003031618.1	Na/Pi cotransporter family protein	-
E6P06_RS04940	310754..311443	-	690	WP_139935893.1	dipeptidase PepE	-
E6P06_RS04945	311657..312016	+	360	WP_003844680.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6P06_RS04950	312006..312323	+	318	WP_003844682.1	helix-turn-helix domain-containing protein	Antitoxin
E6P06_RS04955	312463..312624	-	162	WP_003841416.1	phage protein	-
E6P06_RS27610	312695..312868	-	174	WP_032938222.1	hypothetical protein	-
E6P06_RS04960	313000..313356	+	357	WP_106108076.1	hypothetical protein	-
E6P06_RS04965	313406..314218	-	813	WP_139934991.1	shikimate 5-dehydrogenase	-
E6P06_RS04970	314220..315443	-	1224	WP_139934992.1	L-sorbose 1-phosphate reductase	-
E6P06_RS04975	315511..316335	-	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E6P06_RS04980	316347..317144	-	798	WP_044715019.1	PTS mannose/fructose/sorbose transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13467.51 Da
• Isoelectric Point: 10.1223

>T127514 WP_003844680.1 NZ_CP041051:311657-312016 [Citrobacter sp. CF971]
MTKPLYWVGQARKDLIAMPEHVWDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T127514 NZ_CP041051:311657-312016 [Citrobacter sp. CF971]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTTGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11466.35 Da
• Isoelectric Point: 9.0005

>AT127514 WP_003844682.1 NZ_CP041051:312006-312323 [Citrobacter sp. CF971]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT127514 NZ_CP041051:312006-312323 [Citrobacter sp. CF971]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGGCTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0D7LIR0