Detailed information of TA system TA127513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 150425..151041
• Replicon: chromosome
• Accession: NZ_CP041051
• Organism: Citrobacter sp. CF971

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E6P06_RS04215
• Protein ID: WP_003840448.1
• Coordinates: 150667..151041 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B5NTK4
• Locus tag: E6P06_RS04210
• Protein ID: WP_043018956.1
• Coordinates: 150425..150667 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P06_RS04180	146275..146874	+	600	WP_003028663.1	glucose-1-phosphatase	-
E6P06_RS04185	146868..147740	+	873	WP_139934967.1	virulence factor BrkB family protein	-
E6P06_RS04190	147737..148174	+	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
E6P06_RS04195	148219..149160	+	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
E6P06_RS04205	149311..150219	-	909	WP_003840443.1	alpha/beta hydrolase	-
E6P06_RS04210	150425..150667	+	243	WP_043018956.1	ribbon-helix-helix domain-containing protein	Antitoxin
E6P06_RS04215	150667..151041	+	375	WP_003840448.1	type II toxin-antitoxin system VapC family toxin	Toxin
E6P06_RS04220	151078..152007	-	930	WP_003840450.1	formate dehydrogenase accessory protein FdhE	-
E6P06_RS04225	152004..152639	-	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
E6P06_RS04230	152636..153538	-	903	WP_003847898.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13658.89 Da
• Isoelectric Point: 9.6923

>T127513 WP_003840448.1 NZ_CP041051:150667-151041 [Citrobacter sp. CF971]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEFGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Nucleotide

• Length: 375 bp

>T127513 NZ_CP041051:150667-151041 [Citrobacter sp. CF971]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATTGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATTTGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9451.75 Da
• Isoelectric Point: 4.3437

>AT127513 WP_043018956.1 NZ_CP041051:150425-150667 [Citrobacter sp. CF971]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT127513 NZ_CP041051:150425-150667 [Citrobacter sp. CF971]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6B5NTK4