Detailed information of TA system TA127512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55146..55671
• Replicon: plasmid pBM527-4
• Accession: NZ_CP041050
• Organism: Citrobacter sp. CF971

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: E6P06_RS03315
• Protein ID: WP_139934942.1
• Coordinates: 55366..55671 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: E6P06_RS03310
• Protein ID: WP_001568025.1
• Coordinates: 55146..55364 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P06_RS03290	51050..51583	-	534	WP_048213822.1	hypothetical protein	-
E6P06_RS03295	52246..54456	-	2211	WP_048213823.1	AAA family ATPase	-
E6P06_RS03300	54728..54976	+	249	Protein_63	hypothetical protein	-
E6P06_RS03310	55146..55364	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E6P06_RS03315	55366..55671	+	306	WP_139934942.1	type II toxin-antitoxin system toxin CcdB	Toxin
E6P06_RS03320	55840..56235	+	396	WP_017899885.1	hypothetical protein	-
E6P06_RS03325	56262..56576	+	315	WP_053389906.1	hypothetical protein	-
E6P06_RS03330	56587..57603	+	1017	WP_017899884.1	hypothetical protein	-
E6P06_RS03335	57801..58595	+	795	WP_004197635.1	site-specific integrase	-
E6P06_RS27735	59035..59337	-	303	WP_071571079.1	hypothetical protein	-
E6P06_RS03345	59334..59960	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85170	85170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11599.30 Da
• Isoelectric Point: 6.4672

>T127512 WP_139934942.1 NZ_CP041050:55366-55671 [Citrobacter sp. CF971]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T127512 NZ_CP041050:55366-55671 [Citrobacter sp. CF971]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT127512 WP_001568025.1 NZ_CP041050:55146-55364 [Citrobacter sp. CF971]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT127512 NZ_CP041050:55146-55364 [Citrobacter sp. CF971]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZZP8