Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 46772..47575 Replicon plasmid pBM527-4
Accession NZ_CP041050
Organism Citrobacter sp. CF971

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag E6P06_RS03270 Protein ID WP_048213819.1
Coordinates 47045..47575 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag E6P06_RS03265 Protein ID WP_048213818.1
Coordinates 46772..47041 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6P06_RS03245 43122..43807 + 686 Protein_53 IS3 family transposase -
E6P06_RS03255 44480..45979 + 1500 WP_048213817.1 group II intron reverse transcriptase/maturase -
E6P06_RS03260 46060..46557 + 498 Protein_55 IS3 family transposase -
E6P06_RS03265 46772..47041 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
E6P06_RS03270 47045..47575 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
E6P06_RS03275 47660..48664 - 1005 WP_048213820.1 hypothetical protein -
E6P06_RS03280 48669..48914 - 246 WP_048213821.1 hypothetical protein -
E6P06_RS03285 49673..50695 - 1023 WP_048213854.1 adenylate/guanylate cyclase domain-containing protein -
E6P06_RS03290 51050..51583 - 534 WP_048213822.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..85170 85170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T127511 WP_048213819.1 NZ_CP041050:47045-47575 [Citrobacter sp. CF971]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T127511 NZ_CP041050:47045-47575 [Citrobacter sp. CF971]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGCCAGCACAACAGTAAGATTTTGCGTGGCTATCTGCTCGTGACGAGAGAGG
ATAAACCACGTGTTATGGGGTACTACACGCTGTCTGGCAGCTGCTTTGAGAAAACACTTCTACCCAGCAAAACGCAACAA
AAGCGCGTCCCCTACAAAAATGTGCCGAGCGTGACTTTAGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCTATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGCCTGGGTTTTATACAGTTAAAAGAAGAAAACAGTAATTCGCTC
TTCTACCCGACAAAATCTGTTGAAGAATTGTTCGAAGTAAAAGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT127511 WP_048213818.1 NZ_CP041050:46772-47041 [Citrobacter sp. CF971]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT127511 NZ_CP041050:46772-47041 [Citrobacter sp. CF971]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGATGCAATCACTAATCCGCCAGTACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References