Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 95676..97244 Replicon plasmid pBM527-2
Accession NZ_CP041048
Organism Citrobacter sp. CF971

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag E6P06_RS02420 Protein ID WP_024134513.1
Coordinates 95937..97244 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag E6P06_RS02415 Protein ID WP_039272366.1
Coordinates 95676..95927 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6P06_RS02400 93441..93722 + 282 WP_000575656.1 hypothetical protein -
E6P06_RS02405 93741..94304 + 564 WP_001132404.1 hypothetical protein -
E6P06_RS02410 94307..95311 + 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
E6P06_RS02415 95676..95927 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
E6P06_RS02420 95937..97244 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
E6P06_RS02425 97241..97735 + 495 WP_039272364.1 hypothetical protein -
E6P06_RS02430 97910..98848 - 939 WP_044596315.1 hypothetical protein -
E6P06_RS02435 99047..100687 + 1641 WP_031611419.1 PAAR domain-containing protein -
E6P06_RS02445 101059..101361 - 303 WP_031611417.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / blaCTX-M-3 / blaTEM-1B / blaVIM-1 / aph(3')-XV / ant(3'')-Ia / catB2 / qacE / sul1 / blaSHV-12 htpB 1..151402 151402


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T127510 WP_024134513.1 NZ_CP041048:95937-97244 [Citrobacter sp. CF971]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T127510 NZ_CP041048:95937-97244 [Citrobacter sp. CF971]
ATGCAGACGCTAACAGTGGCGATGAATGGTGAGGTCGTAGGAACACTGTACCGAGATAACAAAGGGGCCATGTCATTTCA
GTATGCCCCGGAGTGGATCTCGGAACCAGGTTCACGCGCCATTTCCATGTCACTGCCTTTGGGTCATGGCAGGATCACAG
GAAGTGAAGTCTTCAATTTCTTTAGCAACCTCCTACCCGACTCAGAGGCAATTATTGCCAGGATGCAGGCTAGGTTCAGA
GTTGAGACAACACATCCATTTGATTTGCTGGCGAGTGTTGGCCGGGATTGTGTTGGAGCTATCCAGCTCTATTCCCCCGA
TGCGGACATCCCAACTGTAATGGAGACGCTTGCAGAGCCTCTGGATGAAGCTCAGATTGAGCAACTGCTTGAAGGCTACC
AGGAAGCGCCCCTTGGGATGGCAGAGGATGCTGATTTTCGGATCTCGATTGCCGGAGCCCAAGAGAAAACTGCACTGCTT
TGGTATCAGGACCGCTGGCAACGCCCCCTGGGCAGCACCCCAACAAGCCATATTTTTAAGCTACCCATTGGTAAGATTGT
TCAAAATAACATCGATCTCAGTGAGAGTTGTGAGAATGAATGGCTCTGTTTGCGCATTGCAAAGGCCTTCGGTTTTGACG
TCGCCAATGCGGACCTAGCAACGTTCGGCAAGAAGAAGGTGCTTGTCGTTCAGCGTTTTGACCGGCGCTGGTCTAAATCC
GGCGAATGGCTTCTACGATTGCCCCAAGAAGACTTCTGCCAAGCTCTTGGGATCGCTCCTGCGCTCAAATACGAGTCACA
TGGAGGGCCAGGTATTGCTGACGCAATGAAGTTGTTGTTGGGGTCCAGACTTGCGGCTAAGGATAGAGAGCAATTTTTCA
AGTCGCAGATCCTATTCTGGATGTTGGCCGCGATTGATGGGCACGGGAAGAACTTCTCCATCTTCATTGAGCCTGCATCG
TCATTTAGGATGACCCCCCTCTACGATGTCATGTCTGCTTTTCCTATCTTCAAAAGCGGGGGAATTGCTGCTCAAAAGGC
CAAGATGGGGATGGCGCTCCAAGGCAAAAACCGCCAATACCATTTTGCGATGATCCAGCCTCGTCATTTTATCAGTACCG
CCGCCCATGTCGGGTTCCCACAAGATACCGCCGCTCAACTTATGTTCGACATGGCTACCAAGACAGAGGAGGTGGTTGCC
ACAGTAGCGGCTGAGCTGCCAGCCGGTTTCCCAGAGCACATCAGTAGCGCCATCTTCGAGGGGCTGTCCAGCCAGGCGGC
TAAGATCTTGAAGGGAGTAGCCAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT127510 WP_039272366.1 NZ_CP041048:95676-95927 [Citrobacter sp. CF971]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT127510 NZ_CP041048:95676-95927 [Citrobacter sp. CF971]
ATGAAAGTGACATCTCCATCCTTGCTTGCGGCTGCGGTGCGTGACCAGCGCAAGCTCAGCAAACTAACGCAGTCAGAGGC
GGCGAAACAGGTCGGAATCAAGCAGACGACCGTCTCTGATTTTGAACTACGGCCAGAATCTACCAAGCTGGAGACGCTAT
TCAAGCTCCTTTCTGCACTGGATCTGGAGCTTCATGTCGTGAAGCGGGGTTCAACATTGGACGATAGTAAAGTCTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References