Detailed information of TA system TA127509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 220938..221488
• Replicon: plasmid pBM527-1
• Accession: NZ_CP041047
• Organism: Citrobacter sp. CF971

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: E6P06_RS01355
• Protein ID: WP_007372286.1
• Coordinates: 220938..221246 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: E6P06_RS01360
• Protein ID: WP_007372285.1
• Coordinates: 221249..221488 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P06_RS01310	215964..216305	-	342	WP_007372296.1	hypothetical protein	-
E6P06_RS01315	216424..216867	-	444	WP_007372295.1	hypothetical protein	-
E6P06_RS01320	217105..217443	+	339	WP_007372294.1	hypothetical protein	-
E6P06_RS01325	217806..218132	-	327	WP_007372293.1	hypothetical protein	-
E6P06_RS01330	218216..218614	-	399	WP_007372292.1	hypothetical protein	-
E6P06_RS01335	218625..218867	-	243	WP_007372291.1	hypothetical protein	-
E6P06_RS01340	219214..219684	-	471	WP_007372290.1	hypothetical protein	-
E6P06_RS01350	220050..220604	-	555	WP_007372288.1	hypothetical protein	-
E6P06_RS27550	220759..220917	-	159	WP_016241558.1	hypothetical protein	-
E6P06_RS01355	220938..221246	-	309	WP_007372286.1	CcdB family protein	Toxin
E6P06_RS01360	221249..221488	-	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E6P06_RS01365	221627..221833	-	207	WP_007372284.1	hypothetical protein	-
E6P06_RS01370	222088..222759	-	672	WP_007372283.1	hypothetical protein	-
E6P06_RS01375	223220..223852	-	633	WP_007372282.1	hypothetical protein	-
E6P06_RS27555	223868..224008	-	141	WP_007372281.1	hypothetical protein	-
E6P06_RS01380	224156..224614	-	459	WP_008786599.1	hypothetical protein	-
E6P06_RS01385	225015..225308	-	294	WP_008786601.1	hypothetical protein	-
E6P06_RS27560	225321..225626	-	306	WP_008786602.1	hypothetical protein	-
E6P06_RS01395	225781..226053	-	273	WP_008786603.1	hypothetical protein	-
E6P06_RS01400	226169..226444	-	276	WP_020996212.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..301036	301036

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T127509 WP_007372286.1 NZ_CP041047:c221246-220938 [Citrobacter sp. CF971]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T127509 NZ_CP041047:c221246-220938 [Citrobacter sp. CF971]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT127509 WP_007372285.1 NZ_CP041047:c221488-221249 [Citrobacter sp. CF971]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT127509 NZ_CP041047:c221488-221249 [Citrobacter sp. CF971]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3