Detailed information of TA system TA127508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 213692..214593
• Replicon: plasmid pBM527-1
• Accession: NZ_CP041047
• Organism: Citrobacter sp. CF971

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: E6P06_RS01290
• Protein ID: WP_007372300.1
• Coordinates: 214135..214593 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: E6P06_RS01285
• Protein ID: WP_008786588.1
• Coordinates: 213692..214138 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P06_RS01260	209728..210570	-	843	WP_007372307.1	HNH endonuclease	-
E6P06_RS27545	211138..211302	-	165	WP_007372306.1	hypothetical protein	-
E6P06_RS01265	211589..212032	-	444	WP_007372305.1	hypothetical protein	-
E6P06_RS01270	212092..212370	-	279	WP_007372304.1	hypothetical protein	-
E6P06_RS01275	212342..213184	-	843	WP_007372303.1	hypothetical protein	-
E6P06_RS01280	213226..213543	-	318	WP_007372302.1	hypothetical protein	-
E6P06_RS01285	213692..214138	+	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
E6P06_RS01290	214135..214593	+	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
E6P06_RS01295	214637..214867	+	231	WP_007372299.1	hypothetical protein	-
E6P06_RS01300	214935..215201	-	267	WP_007372298.1	hypothetical protein	-
E6P06_RS01305	215251..215805	-	555	WP_007372297.1	hypothetical protein	-
E6P06_RS01310	215964..216305	-	342	WP_007372296.1	hypothetical protein	-
E6P06_RS01315	216424..216867	-	444	WP_007372295.1	hypothetical protein	-
E6P06_RS01320	217105..217443	+	339	WP_007372294.1	hypothetical protein	-
E6P06_RS01325	217806..218132	-	327	WP_007372293.1	hypothetical protein	-
E6P06_RS01330	218216..218614	-	399	WP_007372292.1	hypothetical protein	-
E6P06_RS01335	218625..218867	-	243	WP_007372291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..301036	301036

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T127508 WP_007372300.1 NZ_CP041047:214135-214593 [Citrobacter sp. CF971]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T127508 NZ_CP041047:214135-214593 [Citrobacter sp. CF971]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAGCGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT127508 WP_008786588.1 NZ_CP041047:213692-214138 [Citrobacter sp. CF971]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT127508 NZ_CP041047:213692-214138 [Citrobacter sp. CF971]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A837LJP8