Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9181..9824 Replicon plasmid pBM527-1
Accession NZ_CP041047
Organism Citrobacter sp. CF971

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag E6P06_RS00075 Protein ID WP_001044770.1
Coordinates 9408..9824 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag E6P06_RS00070 Protein ID WP_001261282.1
Coordinates 9181..9411 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6P06_RS00035 4329..5026 + 698 Protein_6 IS1 family transposase -
E6P06_RS00040 5105..5802 + 698 Protein_7 IS1 family transposase -
E6P06_RS00045 5995..6525 - 531 WP_039265231.1 hypothetical protein -
E6P06_RS00050 6860..7639 - 780 WP_048213827.1 site-specific integrase -
E6P06_RS00055 7636..8202 - 567 WP_048213826.1 hypothetical protein -
E6P06_RS00060 8265..8624 - 360 WP_048213825.1 hypothetical protein -
E6P06_RS00065 8763..9224 - 462 WP_168199034.1 hypothetical protein -
E6P06_RS00070 9181..9411 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E6P06_RS00075 9408..9824 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
E6P06_RS00080 9898..10134 + 237 WP_001348075.1 AAA family ATPase -
E6P06_RS00085 10181..10885 + 705 WP_059331711.1 IS6-like element IS26 family transposase -
E6P06_RS00090 10831..11646 - 816 WP_139934917.1 ExeA family protein -
E6P06_RS00095 11636..13297 - 1662 WP_033488203.1 IS481 family transposase -
E6P06_RS00100 13281..13841 - 561 WP_021242984.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..301036 301036
- inside IScluster/Tn - - 461..33909 33448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T127507 WP_001044770.1 NZ_CP041047:9408-9824 [Citrobacter sp. CF971]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T127507 NZ_CP041047:9408-9824 [Citrobacter sp. CF971]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT127507 WP_001261282.1 NZ_CP041047:9181-9411 [Citrobacter sp. CF971]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT127507 NZ_CP041047:9181-9411 [Citrobacter sp. CF971]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References