Detailed information of TA system TA127503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 8443..9065
• Replicon: plasmid pAK1
• Accession: NZ_CP041045
• Organism: Paracoccus sp. AK26

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FIU66_RS15860
• Protein ID: WP_167623886.1
• Coordinates: 8443..8838 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FIU66_RS15865
• Protein ID: WP_167623887.1
• Coordinates: 8838..9065 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU66_RS15850	5760..6620	+	861	WP_167623884.1	protein-glutamate O-methyltransferase CheR	-
FIU66_RS15855	6613..8436	+	1824	WP_167623885.1	response regulator	-
FIU66_RS15860	8443..8838	-	396	WP_167623886.1	PIN domain-containing protein	Toxin
FIU66_RS15865	8838..9065	-	228	WP_167623887.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FIU66_RS15870	9108..10022	-	915	WP_167623888.1	recombinase family protein	-
FIU66_RS15875	10892..11992	-	1101	WP_167624256.1	NADH:flavin oxidoreductase	-
FIU66_RS15880	12088..12972	+	885	WP_167623889.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..471727	471727
	-	flank	IS/Tn	-	-	13470..14498	1028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14349.48 Da
• Isoelectric Point: 4.6779

>T127503 WP_167623886.1 NZ_CP041045:c8838-8443 [Paracoccus sp. AK26]
MPGAFFDTNVILYLLDEGPKCDRAEALLAGGGTISVQVLNEALVNCLRKARMTPDEAADFLHGVRQLCTVRDLDLRSHDI
GRALNARHGFSVYDSMIVASALLAGCDILWSEDMQDGMLVEGQLRIRNPFA

Nucleotide

• Length: 396 bp

>T127503 NZ_CP041045:c8838-8443 [Paracoccus sp. AK26]
ATGCCCGGCGCCTTCTTCGACACGAATGTCATCCTCTACCTGCTGGACGAAGGCCCCAAATGCGACCGGGCCGAAGCCCT
GCTGGCCGGGGGCGGCACCATCAGCGTGCAGGTTCTGAACGAGGCCCTGGTCAACTGCCTGCGCAAGGCCCGCATGACCC
CGGACGAGGCCGCGGATTTCCTGCACGGCGTCCGCCAGCTTTGCACGGTGCGGGATCTGGATCTGCGCAGCCATGACATC
GGCCGGGCGCTGAACGCGCGCCATGGCTTTTCGGTCTATGATTCCATGATCGTCGCCTCGGCCCTGCTGGCCGGCTGCGA
CATCCTGTGGTCCGAGGACATGCAGGACGGGATGCTGGTCGAAGGCCAGTTGCGGATCCGCAATCCCTTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8558.74 Da
• Isoelectric Point: 10.9716

>AT127503 WP_167623887.1 NZ_CP041045:c9065-8838 [Paracoccus sp. AK26]
MQVAKWGNSLAVRLPADLVRTLDLREGDHIDLRAEAEGFAVVRQPRPEEILAGLRRFRGRLPAGDRLDRDEANRR

Nucleotide

• Length: 228 bp

>AT127503 NZ_CP041045:c9065-8838 [Paracoccus sp. AK26]
ATGCAAGTCGCTAAATGGGGCAATTCCCTGGCGGTGCGCCTGCCCGCCGACCTGGTGCGGACGCTTGACCTGCGCGAGGG
CGACCATATCGACCTGCGCGCCGAGGCCGAGGGCTTCGCCGTGGTTCGCCAGCCCCGCCCCGAGGAGATCCTGGCCGGGC
TGCGCCGCTTTCGCGGCCGCCTGCCCGCAGGCGACCGGCTTGACCGGGACGAGGCCAACAGGCGCTGA