Detailed information of TA system TA127493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 723548..724077
• Replicon: chromosome
• Accession: NZ_CP041037
• Organism: Staphylococcus aureus strain NP66

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FIV54_RS03670
• Protein ID: WP_000621175.1
• Coordinates: 723715..724077 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FIV54_RS03665
• Protein ID: WP_000948331.1
• Coordinates: 723548..723718 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV54_RS03635	718584..719144	+	561	WP_001092417.1	K(+)-transporting ATPase subunit C	-
FIV54_RS03640	719353..719832	+	480	WP_001287085.1	hypothetical protein	-
FIV54_RS03645	719825..721408	+	1584	WP_001294627.1	PH domain-containing protein	-
FIV54_RS03650	721395..721886	+	492	WP_001205910.1	PH domain-containing protein	-
FIV54_RS03655	721890..722249	+	360	WP_000581197.1	holo-ACP synthase	-
FIV54_RS03660	722315..723463	+	1149	WP_001281154.1	alanine racemase	-
FIV54_RS03665	723548..723718	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FIV54_RS03670	723715..724077	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FIV54_RS03675	724426..725427	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FIV54_RS03680	725546..725872	+	327	WP_001052491.1	anti-sigma factor antagonist	-
FIV54_RS03685	725874..726353	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
FIV54_RS03690	726328..727098	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T127493 WP_000621175.1 NZ_CP041037:723715-724077 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T127493 NZ_CP041037:723715-724077 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT127493 WP_000948331.1 NZ_CP041037:723548-723718 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT127493 NZ_CP041037:723548-723718 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1