Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 102835..103360 Replicon plasmid pLB_CTX-M-15_PT109
Accession NZ_CP041032
Organism Escherichia coli strain PT109

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FIR93_RS23810 Protein ID WP_001159871.1
Coordinates 102835..103140 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FIR93_RS23815 Protein ID WP_000813630.1
Coordinates 103142..103360 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIR93_RS23795 98797..99963 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FIR93_RS23800 100551..101306 - 756 WP_000852146.1 replication initiation protein RepE -
FIR93_RS23805 102028..102834 - 807 WP_000016970.1 site-specific integrase -
FIR93_RS23810 102835..103140 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FIR93_RS23815 103142..103360 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FIR93_RS23820 103920..104150 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FIR93_RS23825 104147..104563 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
FIR93_RS23830 104638..106203 + 1566 WP_001128474.1 AAA family ATPase -
FIR93_RS23835 106188..107210 + 1023 WP_000361402.1 helicase UvrD -
FIR93_RS23840 107464..108150 - 687 Protein_129 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD senB / iutA / iucD / iucC / iucB / iucA 1..126409 126409
- flank IS/Tn - - 107464..107811 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T127480 WP_001159871.1 NZ_CP041032:c103140-102835 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127480 NZ_CP041032:c103140-102835 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT127480 WP_000813630.1 NZ_CP041032:c103360-103142 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127480 NZ_CP041032:c103360-103142 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References