Detailed information of TA system TA127463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1347033..1347658
• Replicon: chromosome
• Accession: NZ_CP041031
• Organism: Escherichia coli strain PT109

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FIR93_RS06480
• Protein ID: WP_000911330.1
• Coordinates: 1347260..1347658 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FIR93_RS06475
• Protein ID: WP_000450524.1
• Coordinates: 1347033..1347260 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIR93_RS06450	1342836..1343306	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FIR93_RS06455	1343306..1343878	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FIR93_RS06460	1344024..1344902	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FIR93_RS06465	1344919..1345953	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FIR93_RS06470	1346166..1346879	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FIR93_RS06475	1347033..1347260	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FIR93_RS06480	1347260..1347658	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FIR93_RS06485	1347805..1348668	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
FIR93_RS06490	1348683..1350698	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FIR93_RS06495	1350772..1351470	+	699	WP_000679823.1	esterase	-
FIR93_RS06500	1351580..1351780	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T127463 WP_000911330.1 NZ_CP041031:1347260-1347658 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T127463 NZ_CP041031:1347260-1347658 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT127463 WP_000450524.1 NZ_CP041031:1347033-1347260 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT127463 NZ_CP041031:1347033-1347260 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33