Detailed information of TA system TA127434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 9976..10510
• Replicon: plasmid pSF3
• Accession: NZ_CP041020
• Organism: Sphingobium fuliginis ATCC 27551

Toxin (Protein)

• Gene name: relE
• Uniprot ID: K4I2N6
• Locus tag: FIL70_RS25265
• Protein ID: WP_004213354.1
• Coordinates: 9976..10257 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K4I0E1
• Locus tag: FIL70_RS25270
• Protein ID: WP_004213356.1
• Coordinates: 10244..10510 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIL70_RS25220	5419..5790	-	372	WP_015063472.1	hypothetical protein	-
FIL70_RS25225	5825..6190	-	366	WP_015063471.1	hypothetical protein	-
FIL70_RS25230	6265..6588	+	324	WP_004213342.1	hypothetical protein	-
FIL70_RS25235	6640..7035	+	396	WP_004213344.1	hypothetical protein	-
FIL70_RS25240	7090..8004	+	915	WP_140043578.1	tyrosine-type recombinase/integrase	-
FIL70_RS25245	8008..8682	+	675	WP_140043575.1	hypothetical protein	-
FIL70_RS25250	8700..8969	+	270	WP_037492522.1	hypothetical protein	-
FIL70_RS25255	8966..9346	-	381	WP_004213350.1	hypothetical protein	-
FIL70_RS25260	9346..9966	-	621	WP_015063469.1	histidine phosphatase family protein	-
FIL70_RS25265	9976..10257	-	282	WP_004213354.1	type II toxin-antitoxin system YafQ family toxin	Toxin
FIL70_RS25270	10244..10510	-	267	WP_004213356.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FIL70_RS25275	10693..11259	-	567	WP_174320780.1	hypothetical protein	-
FIL70_RS25280	11517..11738	+	222	WP_004213362.1	hypothetical protein	-
FIL70_RS25290	12113..12745	+	633	WP_004213363.1	hypothetical protein	-
FIL70_RS25295	12867..13502	+	636	WP_004213364.1	AAA family ATPase	-
FIL70_RS25300	13502..13786	+	285	WP_004213365.1	hypothetical protein	-
FIL70_RS25305	13815..14918	-	1104	WP_004213366.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..43234	43234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10756.14 Da
• Isoelectric Point: 8.0689

>T127434 WP_004213354.1 NZ_CP041020:c10257-9976 [Sphingobium fuliginis ATCC 27551]
MRTIDRSTRFKRDYKRELKGRHRATLDADFQPVLAALATDTPLDPKYRDHDLSGDWAGYRDCHIKPDLVLIYAKPDDETL
RLARLGSHSEVFG

Nucleotide

• Length: 282 bp

>T127434 NZ_CP041020:c10257-9976 [Sphingobium fuliginis ATCC 27551]
ATGCGGACGATTGATCGCTCGACCCGGTTCAAGCGCGACTACAAGCGGGAATTGAAAGGCCGGCACCGGGCGACCCTCGA
CGCCGATTTTCAGCCCGTTCTGGCCGCGCTCGCGACCGACACGCCGCTTGATCCCAAATACCGCGACCATGATCTGAGCG
GCGATTGGGCGGGCTATCGCGATTGCCACATCAAGCCGGACCTGGTGCTGATCTACGCCAAGCCCGACGACGAAACGCTG
CGCCTCGCGCGCCTTGGCTCTCATAGCGAGGTATTCGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9432.88 Da
• Isoelectric Point: 5.8296

>AT127434 WP_004213356.1 NZ_CP041020:c10510-10244 [Sphingobium fuliginis ATCC 27551]
MAVSDTYVRARIDNATKERATAALGAMGLSISDAIRLLMLRIADERRLPFEVKVPNAATREAMAELAAGKGTKFASVEAL
MADLHADD

Nucleotide

• Length: 267 bp

>AT127434 NZ_CP041020:c10510-10244 [Sphingobium fuliginis ATCC 27551]
ATGGCTGTTTCCGACACCTATGTTCGCGCCAGGATCGACAATGCAACGAAGGAACGCGCCACCGCCGCGCTGGGCGCGAT
GGGGCTTTCGATCTCCGACGCGATCCGCCTGCTGATGCTGCGTATCGCCGATGAACGTCGCCTGCCTTTCGAGGTCAAGG
TGCCGAACGCCGCGACGCGCGAGGCAATGGCGGAGCTGGCGGCGGGGAAGGGAACCAAGTTCGCCAGCGTCGAGGCGCTG
ATGGCGGACCTCCATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: K4I2N6

Antitoxin

• AlphaFold DB: K4I0E1