Detailed information of TA system TA127433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 48675..49372
• Replicon: plasmid pSF2
• Accession: NZ_CP041019
• Organism: Sphingobium fuliginis ATCC 27551

Toxin (Protein)

• Gene name: tad
• Uniprot ID: T0J3S2
• Locus tag: FIL70_RS25105
• Protein ID: WP_013041565.1
• Coordinates: 48998..49372 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: T0G937
• Locus tag: FIL70_RS25100
• Protein ID: WP_006964370.1
• Coordinates: 48675..49001 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIL70_RS25070	44274..45029	+	756	WP_022675885.1	P-type conjugative transfer protein TrbJ	-
FIL70_RS25075	45039..45281	+	243	WP_019053956.1	EexN family lipoprotein	-
FIL70_RS25080	45292..47018	+	1727	Protein_46	P-type conjugative transfer protein TrbL	-
FIL70_RS25085	47008..48120	+	1113	WP_013041568.1	muramidase	-
FIL70_RS25090	48141..48440	-	300	WP_013041567.1	hypothetical protein	-
FIL70_RS25095	48433..48675	-	243	WP_013041566.1	hypothetical protein	-
FIL70_RS25100	48675..49001	-	327	WP_006964370.1	helix-turn-helix domain-containing protein	Antitoxin
FIL70_RS25105	48998..49372	-	375	WP_013041565.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIL70_RS25935	49794..49970	+	177	WP_167706880.1	hypothetical protein	-
FIL70_RS25110	49967..50731	-	765	WP_006949122.1	IS6 family transposase	-
FIL70_RS25120	50977..51372	-	396	WP_140043564.1	pyrimidine utilization protein C	-
FIL70_RS25130	51519..52421	-	903	WP_140043566.1	MFS transporter	-
FIL70_RS25135	52459..53919	-	1461	WP_140043567.1	carboxylesterase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60848	60848
	-	flank	IS/Tn	-	-	49967..50731	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (15-94)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13960.67 Da
• Isoelectric Point: 9.4885

>T127433 WP_013041565.1 NZ_CP041019:c49372-48998 [Sphingobium fuliginis ATCC 27551]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA

Nucleotide

• Length: 375 bp

>T127433 NZ_CP041019:c49372-48998 [Sphingobium fuliginis ATCC 27551]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11792.57 Da
• Isoelectric Point: 7.1681

>AT127433 WP_006964370.1 NZ_CP041019:c49001-48675 [Sphingobium fuliginis ATCC 27551]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Nucleotide

• Length: 327 bp

>AT127433 NZ_CP041019:c49001-48675 [Sphingobium fuliginis ATCC 27551]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTGGCCGAGGCCATGCAGGAGCGGAAGTTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTAGCC
ATGCTCACCCGGCTTGGCTGCAAGGTCGATATTGTCGTGCGCCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0J7XHG4

Antitoxin

• AlphaFold DB: A0A0J8A803