Detailed information of TA system TA127430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParST/RES-Xre
• Location: 1279118..1280079
• Replicon: chromosome
• Accession: NZ_CP041016
• Organism: Sphingobium fuliginis ATCC 27551

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: A0A5B8CC27
• Locus tag: FIL70_RS06030
• Protein ID: WP_044662136.1
• Coordinates: 1279118..1279603 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: -
• Locus tag: FIL70_RS06035
• Protein ID: WP_140041837.1
• Coordinates: 1279600..1280079 (-)
• Length: 160 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIL70_RS06005	1274580..1274999	+	420	WP_140041834.1	hypothetical protein	-
FIL70_RS06010	1275239..1275658	+	420	WP_044662132.1	helix-turn-helix domain-containing protein	-
FIL70_RS06015	1275668..1276300	+	633	WP_140041835.1	GntR family transcriptional regulator	-
FIL70_RS06020	1276424..1276558	+	135	WP_137709742.1	benenodin family lasso peptide	-
FIL70_RS25670	1276623..1277279	+	657	WP_167706600.1	lasso peptide biosynthesis B2 protein	-
FIL70_RS06025	1277279..1279036	+	1758	WP_140041836.1	asparagine synthetase B family protein	-
FIL70_RS06030	1279118..1279603	-	486	WP_044662136.1	RES family NAD+ phosphorylase	Toxin
FIL70_RS06035	1279600..1280079	-	480	WP_140041837.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
FIL70_RS06040	1280167..1282251	-	2085	WP_140041838.1	Atxe2 family lasso peptide isopeptidase	-
FIL70_RS06045	1282251..1284815	-	2565	WP_140042969.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1243329..1490228	246899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-149)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17375.85 Da
• Isoelectric Point: 7.1664

>T127430 WP_044662136.1 NZ_CP041016:c1279603-1279118 [Sphingobium fuliginis ATCC 27551]
VTTSFWRIATDARTYEADDLSGAGAKITGGRWNEVGVAIVYAASSRALACLETVVHLNSGGLPLNRYLVEIEVPDEVLAS
AEVATPGNLPVGWDAEPAGRVSISFGSQWAQSQRTALLLVPSVIVPEETNLLINPAHPDAKGIKARKVRKWLYDPRMIRK
A

Nucleotide

• Length: 486 bp

>T127430 NZ_CP041016:c1279603-1279118 [Sphingobium fuliginis ATCC 27551]
GTGACGACAAGCTTCTGGAGAATCGCAACCGACGCCAGGACCTATGAAGCGGACGACCTGTCAGGCGCCGGAGCCAAGAT
CACCGGGGGCCGGTGGAATGAGGTCGGCGTCGCCATCGTCTATGCGGCGAGCAGCCGGGCACTCGCCTGCCTGGAGACGG
TCGTTCATCTGAACTCGGGTGGGTTGCCGCTCAATCGCTACCTCGTCGAAATCGAGGTGCCGGACGAGGTGCTGGCATCT
GCGGAGGTCGCGACACCCGGCAATCTCCCGGTCGGCTGGGATGCCGAACCGGCGGGACGGGTCAGCATCTCATTCGGATC
GCAATGGGCGCAGAGCCAAAGGACAGCGCTGCTGCTCGTCCCTTCCGTCATTGTGCCCGAGGAGACTAATCTCCTCATCA
ATCCCGCGCACCCCGATGCCAAGGGGATCAAGGCCCGGAAAGTGCGCAAATGGCTCTATGATCCAAGGATGATCCGCAAG
GCGTGA

Antitoxin

Protein

• Length: 160 a.a.
• Molecular weight: 17049.47 Da
• Isoelectric Point: 5.2615

>AT127430 WP_140041837.1 NZ_CP041016:c1280079-1279600 [Sphingobium fuliginis ATCC 27551]
MSELETGARKPQGKGTPPFAYKALYRASPVDRIGVIKKGVAASDLKRFIAALHVDQKVMFDALNLKTATVNKKAANNQPL
STEDSERVLGLAKLVGQLEDMVEDSGETDGFDAPEWLSSWLRQPLPALGGVNPIDLLDTMEGQAVVSRALAQIQSGAFA

Nucleotide

• Length: 480 bp

>AT127430 NZ_CP041016:c1280079-1279600 [Sphingobium fuliginis ATCC 27551]
ATGTCCGAACTCGAAACTGGCGCGCGCAAGCCGCAGGGGAAAGGCACGCCCCCCTTTGCCTATAAGGCGCTGTACCGTGC
CTCGCCGGTAGATCGAATCGGCGTCATCAAGAAGGGCGTGGCGGCCAGCGACCTGAAGCGCTTCATCGCCGCCTTGCATG
TGGACCAGAAGGTCATGTTCGACGCGCTCAATCTCAAGACGGCCACCGTGAACAAGAAGGCGGCGAACAACCAGCCGCTT
TCGACGGAGGACAGTGAACGGGTTCTGGGCCTTGCAAAACTGGTCGGCCAGCTCGAAGACATGGTCGAGGATTCGGGCGA
GACGGATGGGTTCGATGCGCCCGAATGGCTCTCGTCCTGGCTGCGTCAGCCTCTCCCGGCGCTGGGAGGCGTCAATCCGA
TCGATCTTCTCGACACGATGGAGGGACAGGCCGTCGTGTCCCGCGCCCTCGCGCAAATCCAGAGCGGCGCCTTTGCGTGA

Structures

Toxin

• PDB: 6D0H
• PDB: 6D0I