Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 944513..945147 Replicon chromosome
Accession NZ_CP041016
Organism Sphingobium fuliginis ATCC 27551

Toxin (Protein)


Gene name vapC Uniprot ID T0IXY4
Locus tag FIL70_RS04370 Protein ID WP_021225100.1
Coordinates 944513..944902 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A5B8CCM4
Locus tag FIL70_RS04375 Protein ID WP_031297643.1
Coordinates 944899..945147 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIL70_RS04350 940471..941877 - 1407 WP_140041721.1 TonB-dependent receptor plug domain-containing protein -
FIL70_RS04355 941940..942896 + 957 WP_152528014.1 AraC family transcriptional regulator -
FIL70_RS04360 943220..943579 + 360 WP_201767462.1 acetyl-CoA acetyltransferase -
FIL70_RS04365 944181..944513 - 333 WP_021225099.1 hypothetical protein -
FIL70_RS04370 944513..944902 - 390 WP_021225100.1 type II toxin-antitoxin system VapC family toxin Toxin
FIL70_RS04375 944899..945147 - 249 WP_031297643.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FIL70_RS04380 945388..945987 - 600 WP_084514710.1 TetR/AcrR family transcriptional regulator -
FIL70_RS04385 946233..946961 + 729 WP_031297645.1 acetoacetyl-CoA reductase -
FIL70_RS04390 947062..947505 + 444 WP_021225104.1 hypothetical protein -
FIL70_RS04395 947588..949057 + 1470 WP_040603655.1 efflux transporter outer membrane subunit -
FIL70_RS04400 949054..950130 + 1077 WP_021225106.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 939210..940229 1019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-121)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13953.80 Da        Isoelectric Point: 4.5908

>T127429 WP_021225100.1 NZ_CP041016:c944902-944513 [Sphingobium fuliginis ATCC 27551]
MILDTSALVAILYREPEAESFTQAIHNADACRISGATYVELSIVIENQLGSEGMRQAEAFFRRAGITIEPVTVEHGELAR
QAFLDFGKGRHKAGLNFGDCFSFALAKATGEPLLFKGNDFALTDLEAAA

Download         Length: 390 bp

>T127429 NZ_CP041016:c944902-944513 [Sphingobium fuliginis ATCC 27551]
ATGATCCTTGATACATCGGCGCTGGTTGCGATCCTTTATCGCGAACCCGAAGCGGAATCGTTTACCCAGGCAATCCACAA
TGCCGACGCATGCCGTATCAGCGGCGCAACCTATGTCGAATTGTCGATCGTCATTGAAAACCAGCTCGGATCCGAAGGAA
TGCGCCAGGCAGAAGCCTTCTTCCGGCGCGCGGGAATTACGATCGAACCGGTGACGGTCGAACATGGTGAGTTGGCGCGG
CAAGCCTTTCTGGATTTCGGCAAGGGCCGCCACAAGGCCGGCTTGAATTTCGGCGACTGCTTTTCATTTGCACTCGCCAA
GGCGACCGGCGAGCCCTTGCTGTTCAAGGGCAATGACTTTGCCCTGACAGATCTCGAGGCGGCTGCATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9169.50 Da        Isoelectric Point: 9.6257

>AT127429 WP_031297643.1 NZ_CP041016:c945147-944899 [Sphingobium fuliginis ATCC 27551]
VSLNVKDPEAHRLARAIAQATGQSMTRVVTEALRECYARIERNKRKASVAELLAIADRAAVHVKRSYIEHGELLYDENGL
PK

Download         Length: 249 bp

>AT127429 NZ_CP041016:c945147-944899 [Sphingobium fuliginis ATCC 27551]
GTGAGCCTTAACGTCAAAGATCCCGAGGCTCATAGACTCGCCCGTGCCATCGCCCAGGCGACCGGCCAGAGCATGACCCG
CGTGGTGACGGAGGCGCTGCGCGAGTGCTACGCCCGGATCGAGCGGAACAAACGCAAGGCAAGCGTGGCGGAGCTGCTGG
CAATCGCGGATCGCGCCGCCGTCCATGTGAAGCGATCCTATATCGAGCATGGCGAGCTGCTTTACGATGAAAATGGCTTG
CCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B8CAU4

Antitoxin

Source ID Structure
AlphaFold DB A0A5B8CCM4

References