Detailed information of TA system TA127422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1329668..1330254
• Replicon: chromosome
• Accession: NZ_CP041013
• Organism: Pseudomonas aeruginosa strain FDAARGOS_610

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: FIU24_RS06370
• Protein ID: WP_003120987.1
• Coordinates: 1329668..1329967 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FIU24_RS06375
• Protein ID: WP_003448662.1
• Coordinates: 1329964..1330254 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU24_RS06350	1324781..1325314	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
FIU24_RS06355	1325304..1326629	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
FIU24_RS06360	1326633..1328342	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
FIU24_RS06365	1328342..1329466	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
FIU24_RS06370	1329668..1329967	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU24_RS06375	1329964..1330254	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
FIU24_RS06380	1330325..1330669	-	345	WP_016851612.1	hypothetical protein	-
FIU24_RS06385	1330810..1332786	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
FIU24_RS06390	1332783..1334672	-	1890	WP_016851610.1	hypothetical protein	-
FIU24_RS06395	1334894..1335103	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1254133..1357505	103372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T127422 WP_003120987.1 NZ_CP041013:1329668-1329967 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T127422 NZ_CP041013:1329668-1329967 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT127422 WP_003448662.1 NZ_CP041013:1329964-1330254 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT127422 NZ_CP041013:1329964-1330254 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73