Detailed information of TA system TA127418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1363840..1364533
• Replicon: chromosome
• Accession: NZ_CP041012
• Organism: Enterococcus faecalis strain FDAARGOS_611

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A2Z6BTE2
• Locus tag: FIU23_RS06820
• Protein ID: WP_002364356.1
• Coordinates: 1363840..1364184 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: FIU23_RS06825
• Protein ID: WP_002364355.1
• Coordinates: 1364201..1364533 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU23_RS06785	1358911..1359957	+	1047	WP_002356990.1	type II secretion system F family protein	-
FIU23_RS06790	1359957..1360232	+	276	WP_002356991.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
FIU23_RS06795	1360229..1360672	+	444	WP_137007137.1	type II secretion system GspH family protein	-
FIU23_RS06800	1360700..1361848	-	1149	WP_002364359.1	site-specific integrase	-
FIU23_RS06805	1361945..1362673	-	729	WP_002364358.1	ion transporter	-
FIU23_RS06815	1362841..1363746	-	906	WP_016630909.1	DUF4352 domain-containing protein	-
FIU23_RS06820	1363840..1364184	-	345	WP_002364356.1	ImmA/IrrE family metallo-endopeptidase	Toxin
FIU23_RS06825	1364201..1364533	-	333	WP_002364355.1	helix-turn-helix domain-containing protein	Antitoxin
FIU23_RS06830	1364845..1365021	+	177	WP_002364354.1	helix-turn-helix transcriptional regulator	-
FIU23_RS06835	1365032..1365330	+	299	Protein_1242	hypothetical protein	-
FIU23_RS06840	1365369..1366094	+	726	WP_002364352.1	phage regulatory protein	-
FIU23_RS06845	1366120..1366308	-	189	WP_002364350.1	YegP family protein	-
FIU23_RS06850	1366363..1366572	+	210	WP_002399426.1	hypothetical protein	-
FIU23_RS06855	1366609..1366947	+	339	WP_002364347.1	hypothetical protein	-
FIU23_RS06865	1367232..1367786	-	555	WP_002357006.1	hypothetical protein	-
FIU23_RS06870	1368006..1368323	+	318	WP_002357007.1	hypothetical protein	-
FIU23_RS06875	1368316..1369050	+	735	WP_002364345.1	ERF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13704.62 Da
• Isoelectric Point: 5.8535

>T127418 WP_002364356.1 NZ_CP041012:c1364184-1363840 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYMELYKIPSFRNKMEAEADH
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Nucleotide

• Length: 345 bp

>T127418 NZ_CP041012:c1364184-1363840 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCGGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAAGAAACTTATTTAAATTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12691.16 Da
• Isoelectric Point: 4.6307

>AT127418 WP_002364355.1 NZ_CP041012:c1364533-1364201 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT127418 NZ_CP041012:c1364533-1364201 [Enterococcus faecalis]
ATGAACCTCTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Structures

Toxin

• AlphaFold DB: A0A2Z6BTE2