Detailed information of TA system TA127412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 79286..80319
• Replicon: chromosome
• Accession: NZ_CP041012
• Organism: Enterococcus faecalis strain FDAARGOS_611

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: R3K9G6
• Locus tag: FIU23_RS00565
• Protein ID: WP_002365229.1
• Coordinates: 79666..80319 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: FIU23_RS00560
• Protein ID: WP_002365228.1
• Coordinates: 79286..79669 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU23_RS00510	75115..75483	-	369	WP_002365216.1	DUF86 domain-containing protein	-
FIU23_RS00515	75467..75799	-	333	WP_002399122.1	nucleotidyltransferase domain-containing protein	-
FIU23_RS00520	75859..76182	-	324	WP_002399121.1	hypothetical protein	-
FIU23_RS00525	76222..76461	-	240	WP_002365219.1	hypothetical protein	-
FIU23_RS00530	76472..76624	-	153	WP_002365221.1	hypothetical protein	-
FIU23_RS00535	76651..76875	-	225	WP_002365222.1	hypothetical protein	-
FIU23_RS00540	77107..77295	+	189	WP_002365223.1	hypothetical protein	-
FIU23_RS14475	77282..77443	-	162	WP_002365224.1	hypothetical protein	-
FIU23_RS00545	77466..77882	-	417	WP_002365225.1	DUF961 family protein	-
FIU23_RS00550	77882..78208	-	327	WP_002365226.1	hypothetical protein	-
FIU23_RS00555	78294..78545	-	252	WP_002365227.1	hypothetical protein	-
FIU23_RS00560	79286..79669	+	384	WP_002365228.1	helix-turn-helix transcriptional regulator	Antitoxin
FIU23_RS00565	79666..80319	+	654	WP_002365229.1	ImmA/IrrE family metallo-endopeptidase	Toxin
FIU23_RS00570	80358..80693	+	336	WP_002365231.1	helix-turn-helix transcriptional regulator	-
FIU23_RS00575	80731..82062	-	1332	WP_002365232.1	FAD-dependent oxidoreductase	-
FIU23_RS00580	82161..83096	-	936	WP_171803825.1	aldo/keto reductase	-
FIU23_RS00585	83111..83530	-	420	WP_002365234.1	MerR family transcriptional regulator	-
FIU23_RS00590	83547..84128	-	582	WP_002365235.1	phosphoglycerate mutase family protein	-
FIU23_RS00595	84350..84679	+	330	WP_002365236.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (80-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26065.01 Da
• Isoelectric Point: 6.2394

>T127412 WP_002365229.1 NZ_CP041012:79666-80319 [Enterococcus faecalis]
MIGLLLVDFISKELYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T127412 NZ_CP041012:79666-80319 [Enterococcus faecalis]
TTGATTGGACTGTTGTTGGTGGATTTTATTAGTAAAGAACTCTATTTTGAAGCATTTAATTTTTCGAACGAATTAATAAA
AGAAGTATCTTACTACTCTTCCAAAAATATTGAACAAGTTAAATGCTTTGATATTGAAAAATATGTAAAAGAAGTTGAAG
ATGTTGAATTTGTTGAATACACGTTTCAAAAACAACTTAAAAGACGGATGCTTGGTTCAATCTCTAAAGTAGACGATGAA
GTCATTATAACAACAAACAAAGAATTAATGTTAGAACGAAAAAATTTCACTAAAATGCATGAGGTTATGCATTACTACAT
AGATATACCTAAAATTAATAATGCAACTCATACTTTTTCTGATATTCTTTTGAAAAATGGTTACTTGATGGAAGATTTTC
CAAAAGAATATCGTGCTAATGTCGGTGCTAGTATGTTAATGGCAAATGATCAAGCATTGTTTTACGCATTAAAAAAATTT
TATTCATTCGAAGAAATTGCACAATACTTTTTTATGAGCAAATCTGCATTAAGAAATAGACTCATCGAGCATCTTATGTA
TGTAAATAATTGTACTTTTGCACACGCTAATACACTTTTTAACAATTATTACTTTCATAAAGAAACTGATATCTACAGAT
TTATTTTCAATTAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 15032.03 Da
• Isoelectric Point: 5.1063

>AT127412 WP_002365228.1 NZ_CP041012:79286-79669 [Enterococcus faecalis]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMDIEDIEELEEELIDFQDFLIKKAKEKKERNKKN

Nucleotide

• Length: 384 bp

>AT127412 NZ_CP041012:79286-79669 [Enterococcus faecalis]
GTGCAACCATTAGCAAAAAGACTACAGCTTTTAAGAGAAGAAAAAGAATGGACAAAAACATATGTTGCAAAACAACTAGG
TTTAAATAACTTAGGAACCTATGCAAACTGGGAATATGGAACACGTGAACCAGATTCGGAAATGCTATCAAAAATTGCTT
CCTTATATGATGTATCTACCGATTTTTTACTAGGTAGAACAGAGGAAAGAATACATCTAAACGACAAGGAAAAAATTGGA
AAAAATATCATTTCTCATTTTCGTTTAAATACTTCTGATATGGACATAGAAGATATAGAAGAATTAGAAGAAGAATTAAT
CGACTTTCAAGATTTTCTTATAAAAAAAGCAAAAGAAAAAAAGGAACGTAACAAAAAAAATTGA

Structures

Toxin

• AlphaFold DB: R3K9G6