Detailed information of TA system TA127390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 21927..22462
• Replicon: chromosome
• Accession: NZ_CP041010
• Organism: Staphylococcus aureus strain FDAARGOS_766

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: A0A4P7P340
• Locus tag: FIU19_RS00355
• Protein ID: WP_001103945.1
• Coordinates: 22139..22462 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: FIU19_RS00350
• Protein ID: WP_001058486.1
• Coordinates: 21927..22136 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU19_RS00310	17055..17558	+	504	WP_000934799.1	single-stranded DNA-binding protein	-
FIU19_RS00315	17610..17852	+	243	WP_000897044.1	30S ribosomal protein S18	-
FIU19_RS00320	18084..18878	-	795	WP_001800370.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FIU19_RS00325	18916..20130	-	1215	WP_140045509.1	site-specific integrase	-
FIU19_RS00330	20394..21119	-	726	WP_000668215.1	helix-turn-helix transcriptional regulator	-
FIU19_RS00335	21291..21503	+	213	WP_001245562.1	helix-turn-helix transcriptional regulator	-
FIU19_RS00340	21504..21776	+	273	WP_001138300.1	helix-turn-helix domain-containing protein	-
FIU19_RS00345	21788..21934	+	147	WP_000784875.1	hypothetical protein	-
FIU19_RS00350	21927..22136	+	210	WP_001058486.1	hypothetical protein	Antitoxin
FIU19_RS00355	22139..22462	+	324	WP_001103945.1	DUF1474 family protein	Toxin
FIU19_RS00360	22527..23396	+	870	WP_031765319.1	primase alpha helix C-terminal domain-containing protein	-
FIU19_RS00365	23413..24870	+	1458	WP_031765320.1	virulence-associated E family protein	-
FIU19_RS00370	25171..25533	+	363	WP_031765322.1	hypothetical protein	-
FIU19_RS00375	25535..25819	+	285	WP_000998179.1	hypothetical protein	-
FIU19_RS00380	25816..26457	+	642	WP_140045510.1	pathogenicity island protein	-
FIU19_RS00390	27019..27360	+	342	WP_001190614.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M)	-	750..102690	101940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12665.26 Da
• Isoelectric Point: 4.7124

>T127390 WP_001103945.1 NZ_CP041010:22139-22462 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLQVYLKEFDELIQKF
HEIEKASSDVSLATESDDAKNSIKVAE

Nucleotide

• Length: 324 bp

>T127390 NZ_CP041010:22139-22462 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCGTCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGA
GTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT127390 WP_001058486.1 NZ_CP041010:21927-22136 [Staphylococcus aureus]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT127390 NZ_CP041010:21927-22136 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7P340