Detailed information of TA system TA127376

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4363174..4363975
• Replicon: chromosome
• Accession: NZ_CP041002
• Organism: Escherichia coli strain FDAARGOS_772

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: FIU16_RS22545
• Protein ID: WP_001094437.1
• Coordinates: 4363598..4363975 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: FIU16_RS22540
• Protein ID: WP_001390338.1
• Coordinates: 4363174..4363551 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU16_RS22505	4360292..4360450	+	159	WP_001323397.1	DUF905 family protein	-
FIU16_RS22515	4360605..4361423	+	819	WP_001234716.1	DUF945 domain-containing protein	-
FIU16_RS27430	4361423..4361596	+	174	WP_001183321.1	hypothetical protein	-
FIU16_RS22525	4361762..4362235	+	474	WP_001387789.1	antirestriction protein	-
FIU16_RS22530	4362251..4362727	+	477	WP_001186773.1	RadC family protein	-
FIU16_RS22535	4362790..4363011	+	222	WP_000692300.1	DUF987 domain-containing protein	-
FIU16_RS22540	4363174..4363551	+	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FIU16_RS22545	4363598..4363975	+	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
FIU16_RS22550	4363972..4364460	+	489	WP_000761716.1	hypothetical protein	-
FIU16_RS22555	4364480..4364677	+	198	WP_000772032.1	DUF957 domain-containing protein	-
FIU16_RS22560	4364762..4365607	+	846	WP_001274561.1	DUF4942 domain-containing protein	-
FIU16_RS22570	4366400..4367584	+	1185	WP_001172875.1	sugar efflux transporter	-
FIU16_RS22575	4367695..4368618	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T127376 WP_001094437.1 NZ_CP041002:4363598-4363975 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T127376 NZ_CP041002:4363598-4363975 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT127376 WP_001390338.1 NZ_CP041002:4363174-4363551 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT127376 NZ_CP041002:4363174-4363551 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1