Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3411587..3412241 Replicon chromosome
Accession NZ_CP041002
Organism Escherichia coli strain FDAARGOS_772

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag FIU16_RS17710 Protein ID WP_000244772.1
Coordinates 3411587..3411994 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FIU16_RS17715 Protein ID WP_000354046.1
Coordinates 3411975..3412241 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIU16_RS17690 3407544..3409277 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
FIU16_RS17695 3409283..3409993 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FIU16_RS17700 3410018..3410914 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FIU16_RS17705 3411026..3411547 + 522 WP_001055874.1 flavodoxin FldB -
FIU16_RS17710 3411587..3411994 - 408 WP_000244772.1 protein YgfX Toxin
FIU16_RS17715 3411975..3412241 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FIU16_RS17720 3412484..3413464 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FIU16_RS17725 3413542..3414201 - 660 WP_000250274.1 hemolysin III family protein -
FIU16_RS17730 3414365..3414676 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FIU16_RS17735 3414721..3416154 + 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
FIU16_RS17740 3416211..3416954 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T127373 WP_000244772.1 NZ_CP041002:c3411994-3411587 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T127373 NZ_CP041002:c3411994-3411587 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT127373 WP_000354046.1 NZ_CP041002:c3412241-3411975 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT127373 NZ_CP041002:c3412241-3411975 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References