Detailed information of TA system TA127360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1327875..1328356
• Replicon: chromosome
• Accession: NZ_CP041002
• Organism: Escherichia coli strain FDAARGOS_772

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7YS45
• Locus tag: FIU16_RS07070
• Protein ID: WP_014966210.1
• Coordinates: 1327875..1328165 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0D7Y007
• Locus tag: FIU16_RS07075
• Protein ID: WP_000100896.1
• Coordinates: 1328165..1328356 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU16_RS07035	1323118..1323387	-	270	WP_000003742.1	excisionase	-
FIU16_RS07040	1323449..1325887	-	2439	WP_000102130.1	exonuclease	-
FIU16_RS07045	1325980..1326168	-	189	WP_000199475.1	DUF1482 family protein	-
FIU16_RS07050	1326165..1326353	-	189	WP_000449175.1	cell division inhibitor	-
FIU16_RS07055	1326848..1327114	+	267	WP_012601410.1	hypothetical protein	-
FIU16_RS07060	1327103..1327441	-	339	WP_000394541.1	hypothetical protein	-
FIU16_RS07065	1327453..1327605	-	153	WP_000379548.1	DUF1391 family protein	-
FIU16_RS07070	1327875..1328165	-	291	WP_014966210.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU16_RS07075	1328165..1328356	-	192	WP_000100896.1	hypothetical protein	Antitoxin
FIU16_RS07080	1328374..1328874	-	501	WP_000824162.1	helix-turn-helix transcriptional regulator	-
FIU16_RS07085	1328982..1329257	+	276	WP_001048458.1	helix-turn-helix domain-containing protein	-
FIU16_RS07090	1329241..1329666	+	426	WP_000693845.1	Rha family transcriptional regulator	-
FIU16_RS07095	1329689..1330651	+	963	WP_000095671.1	helix-turn-helix domain-containing protein	-
FIU16_RS07100	1330692..1331114	+	423	WP_000416574.1	DUF977 family protein	-
FIU16_RS07105	1331111..1331605	+	495	WP_001275731.1	class I SAM-dependent methyltransferase	-
FIU16_RS07110	1331592..1332278	+	687	WP_000034251.1	ead/Ea22-like family protein	-
FIU16_RS07115	1332275..1333024	+	750	WP_001289900.1	ead/Ea22-like family protein	-
FIU16_RS07120	1333026..1333204	+	179	Protein_1267	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1317763..1370226	52463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11176.05 Da
• Isoelectric Point: 9.9854

>T127360 WP_014966210.1 NZ_CP041002:c1328165-1327875 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Nucleotide

• Length: 291 bp

>T127360 NZ_CP041002:c1328165-1327875 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGTGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGACAGTATCCAAACAAAACCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7033.83 Da
• Isoelectric Point: 4.0270

>AT127360 WP_000100896.1 NZ_CP041002:c1328356-1328165 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRTKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Nucleotide

• Length: 192 bp

>AT127360 NZ_CP041002:c1328356-1328165 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACAGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAAC
CAAAGTGGCAGCAAGCCTCTCAGATCCCCGTCCTGCAATTCCACATGACGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTGCAACGAACAGGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0D7YS45

Antitoxin

• AlphaFold DB: A0A0D7Y007