Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 44319..44844 Replicon plasmid unnamed1
Accession NZ_CP041001
Organism Escherichia coli strain FDAARGOS_772

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag FIU16_RS00290 Protein ID WP_001159872.1
Coordinates 44539..44844 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag FIU16_RS00285 Protein ID WP_000813638.1
Coordinates 44319..44537 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIU16_RS00250 39503..39628 + 126 WP_032159539.1 hypothetical protein -
FIU16_RS00255 39749..40489 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
FIU16_RS00260 40768..41745 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
FIU16_RS00270 42837..42986 + 150 Protein_45 transposase domain-containing protein -
FIU16_RS00275 43069..43470 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
FIU16_RS00280 43494..43724 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FIU16_RS00285 44319..44537 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FIU16_RS00290 44539..44844 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
FIU16_RS00295 44845..45651 + 807 WP_000016961.1 site-specific integrase -
FIU16_RS00300 45830..46474 + 645 WP_001144032.1 ParA family protein -
FIU16_RS00305 46561..46869 + 309 WP_000030204.1 molecular chaperone GroEL -
FIU16_RS00310 47283..48263 + 981 WP_000688504.1 plasmid segregation protein ParM -
FIU16_RS00315 48256..48672 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aggD / aggC / aggB / aggA / aap/aspU 1..75555 75555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T127355 WP_001159872.1 NZ_CP041001:44539-44844 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T127355 NZ_CP041001:44539-44844 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT127355 WP_000813638.1 NZ_CP041001:44319-44537 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127355 NZ_CP041001:44319-44537 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References