Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43069..43724 Replicon plasmid unnamed1
Accession NZ_CP041001
Organism Escherichia coli strain FDAARGOS_772

Toxin (Protein)


Gene name vagD Uniprot ID A0A2H3M3N3
Locus tag FIU16_RS00275 Protein ID WP_001392130.1
Coordinates 43069..43470 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A0E0YAG4
Locus tag FIU16_RS00280 Protein ID WP_001261288.1
Coordinates 43494..43724 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIU16_RS00230 38207..38491 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein -
FIU16_RS00235 38491..38766 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin -
FIU16_RS00240 38772..39103 + 332 Protein_40 type II toxin-antitoxin system ParD family antitoxin -
FIU16_RS00245 39219..39380 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
FIU16_RS00250 39503..39628 + 126 WP_032159539.1 hypothetical protein -
FIU16_RS00255 39749..40489 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
FIU16_RS00260 40768..41745 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
FIU16_RS00270 42837..42986 + 150 Protein_45 transposase domain-containing protein -
FIU16_RS00275 43069..43470 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin Toxin
FIU16_RS00280 43494..43724 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FIU16_RS00285 44319..44537 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA -
FIU16_RS00290 44539..44844 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB -
FIU16_RS00295 44845..45651 + 807 WP_000016961.1 site-specific integrase -
FIU16_RS00300 45830..46474 + 645 WP_001144032.1 ParA family protein -
FIU16_RS00305 46561..46869 + 309 WP_000030204.1 molecular chaperone GroEL -
FIU16_RS00310 47283..48263 + 981 WP_000688504.1 plasmid segregation protein ParM -
FIU16_RS00315 48256..48672 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aggD / aggC / aggB / aggA / aap/aspU 1..75555 75555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14647.00 Da        Isoelectric Point: 6.7123

>T127354 WP_001392130.1 NZ_CP041001:c43470-43069 [Escherichia coli]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 402 bp

>T127354 NZ_CP041001:c43470-43069 [Escherichia coli]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8684.76 Da        Isoelectric Point: 4.3654

>AT127354 WP_001261288.1 NZ_CP041001:c43724-43494 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Download         Length: 231 bp

>AT127354 NZ_CP041001:c43724-43494 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H3M3N3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0YAG4

References