Detailed information of TA system TA127349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2407565..2408094
• Replicon: chromosome
• Accession: NZ_CP040998
• Organism: Staphylococcus aureus strain FDAARGOS_773

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FIU14_RS12075
• Protein ID: WP_000621175.1
• Coordinates: 2407732..2408094 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FIU14_RS12070
• Protein ID: WP_000948331.1
• Coordinates: 2407565..2407735 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU14_RS12040	2402601..2403161	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
FIU14_RS12045	2403370..2403849	+	480	WP_001287088.1	hypothetical protein	-
FIU14_RS12050	2403842..2405425	+	1584	WP_001294626.1	PH domain-containing protein	-
FIU14_RS12055	2405412..2405903	+	492	WP_001205910.1	PH domain-containing protein	-
FIU14_RS12060	2405907..2406266	+	360	WP_000581200.1	holo-ACP synthase	-
FIU14_RS12065	2406332..2407480	+	1149	WP_001281145.1	alanine racemase	-
FIU14_RS12070	2407565..2407735	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FIU14_RS12075	2407732..2408094	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FIU14_RS12080	2408442..2409443	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FIU14_RS12085	2409562..2409888	+	327	WP_001052491.1	anti-sigma factor antagonist	-
FIU14_RS12090	2409890..2410369	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
FIU14_RS12095	2410344..2411114	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T127349 WP_000621175.1 NZ_CP040998:2407732-2408094 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T127349 NZ_CP040998:2407732-2408094 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT127349 WP_000948331.1 NZ_CP040998:2407565-2407735 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT127349 NZ_CP040998:2407565-2407735 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1