Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15453..15978 Replicon plasmid unnamed2
Accession NZ_CP040994
Organism Klebsiella pneumoniae strain FDAARGOS_775

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag FIU12_RS25955 Protein ID WP_013023785.1
Coordinates 15453..15758 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A378F352
Locus tag FIU12_RS25960 Protein ID WP_025862266.1
Coordinates 15760..15978 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIU12_RS25925 12331..12708 + 378 WP_162985544.1 M15 family metallopeptidase -
FIU12_RS25930 12906..13061 + 156 WP_004150747.1 Hok/Gef family protein -
FIU12_RS25935 13185..13679 + 495 Protein_12 helix-turn-helix domain-containing protein -
FIU12_RS25940 13801..13896 + 96 Protein_13 transposase domain-containing protein -
FIU12_RS25945 14403..15197 - 795 WP_025862268.1 site-specific integrase -
FIU12_RS28700 15190..15423 - 234 WP_025862267.1 hypothetical protein -
FIU12_RS25955 15453..15758 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
FIU12_RS25960 15760..15978 - 219 WP_025862266.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FIU12_RS25970 16291..16494 + 204 WP_004150739.1 hemolysin expression modulator Hha -
FIU12_RS25975 16543..16800 + 258 WP_004098928.1 hypothetical protein -
FIU12_RS25980 16859..17113 + 255 WP_025862265.1 hypothetical protein -
FIU12_RS25985 17310..17588 + 279 Protein_21 transposase -
FIU12_RS25990 18237..18797 - 561 Protein_22 porphobilinogen synthase -
FIU12_RS25995 18788..18970 - 183 Protein_23 hypothetical protein -
FIU12_RS26000 18957..19521 - 565 Protein_24 threonine--tRNA ligase -
FIU12_RS26010 19748..20692 - 945 WP_004150724.1 GTP cyclohydrolase I FolE2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - iroB / vat 1..96085 96085
- flank IS/Tn - - 13185..13697 512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T127336 WP_013023785.1 NZ_CP040994:c15758-15453 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127336 NZ_CP040994:c15758-15453 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACAGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8420.39 Da        Isoelectric Point: 4.7405

>AT127336 WP_025862266.1 NZ_CP040994:c15978-15760 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISVLVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127336 NZ_CP040994:c15978-15760 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGTCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A378F352

References