Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2537210..2537829 Replicon chromosome
Accession NZ_CP040993
Organism Klebsiella pneumoniae strain FDAARGOS_775

Toxin (Protein)


Gene name Hha Uniprot ID A0A377W829
Locus tag FIU12_RS12620 Protein ID WP_025861658.1
Coordinates 2537210..2537428 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FIU12_RS12625 Protein ID WP_002892066.1
Coordinates 2537455..2537829 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIU12_RS12590 2533257..2533520 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
FIU12_RS12595 2533520..2533660 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FIU12_RS12600 2533657..2534355 - 699 WP_025861660.1 GNAT family N-acetyltransferase -
FIU12_RS12605 2534456..2535907 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FIU12_RS12610 2535882..2536352 - 471 WP_002892026.1 YlaC family protein -
FIU12_RS27970 2536373..2536513 + 141 WP_004147370.1 hypothetical protein -
FIU12_RS12615 2536485..2537051 - 567 WP_032443798.1 maltose O-acetyltransferase -
FIU12_RS12620 2537210..2537428 - 219 WP_025861658.1 hemolysin expression modulator Hha Toxin
FIU12_RS12625 2537455..2537829 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FIU12_RS12630 2538315..2541461 - 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit -
FIU12_RS12635 2541484..2542677 - 1194 WP_004147376.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8644.00 Da        Isoelectric Point: 8.9008

>T127325 WP_025861658.1 NZ_CP040993:c2537428-2537210 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELSVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T127325 NZ_CP040993:c2537428-2537210 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGTCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT127325 WP_002892066.1 NZ_CP040993:c2537829-2537455 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT127325 NZ_CP040993:c2537829-2537455 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377W829

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References