Detailed information of TA system TA127317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 16233..16707
• Replicon: plasmid unnamed1
• Accession: NZ_CP040992
• Organism: Klebsiella pneumoniae strain FDAARGOS_775

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: FIU12_RS00125
• Protein ID: WP_000262326.1
• Coordinates: 16441..16707 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: FIU12_RS00120
• Protein ID: WP_000879771.1
• Coordinates: 16233..16457 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU12_RS00085	11774..12088	-	315	WP_001136729.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FIU12_RS00090	12076..12354	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	-
FIU12_RS00095	12717..12935	-	219	WP_002193751.1	TonB family protein	-
FIU12_RS00100	12932..13303	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
FIU12_RS00105	13375..14316	-	942	Protein_19	transposase	-
FIU12_RS00110	14303..14890	-	588	Protein_20	DEAD/DEAH box helicase	-
FIU12_RS00115	14918..15493	-	576	WP_000904911.1	recombinase family protein	-
FIU12_RS00120	16233..16457	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
FIU12_RS00125	16441..16707	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU12_RS00130	16764..17005	-	242	Protein_24	hypothetical protein	-
FIU12_RS00135	17332..19020	+	1689	WP_000012966.1	cloacin	-
FIU12_RS00140	19030..19287	+	258	WP_000523382.1	cloacin immunity family protein	-
FIU12_RS00145	19372..19521	+	150	WP_002193747.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26723	26723
	-	flank	IS/Tn	-	-	13375..14319	944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T127317 WP_000262326.1 NZ_CP040992:16441-16707 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T127317 NZ_CP040992:16441-16707 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT127317 WP_000879771.1 NZ_CP040992:16233-16457 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT127317 NZ_CP040992:16233-16457 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACCTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCATCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5