Detailed information of TA system TA127316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 11774..12354
• Replicon: plasmid unnamed1
• Accession: NZ_CP040992
• Organism: Klebsiella pneumoniae strain FDAARGOS_775

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A060VNB1
• Locus tag: FIU12_RS00085
• Protein ID: WP_001136729.1
• Coordinates: 11774..12088 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: FIU12_RS00090
• Protein ID: WP_000093040.1
• Coordinates: 12076..12354 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU12_RS00060	7721..9681	+	1961	Protein_10	TraM recognition domain-containing protein	-
FIU12_RS00065	9681..10412	+	732	WP_032444125.1	replication-relaxation family protein	-
FIU12_RS00070	10419..10949	+	531	WP_001240350.1	hypothetical protein	-
FIU12_RS00075	10977..11156	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
FIU12_RS00080	11182..11610	-	429	WP_001140599.1	hypothetical protein	-
FIU12_RS00085	11774..12088	-	315	WP_001136729.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU12_RS00090	12076..12354	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
FIU12_RS00095	12717..12935	-	219	WP_002193751.1	TonB family protein	-
FIU12_RS00100	12932..13303	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
FIU12_RS00105	13375..14316	-	942	Protein_19	transposase	-
FIU12_RS00110	14303..14890	-	588	Protein_20	DEAD/DEAH box helicase	-
FIU12_RS00115	14918..15493	-	576	WP_000904911.1	recombinase family protein	-
FIU12_RS00120	16233..16457	+	225	WP_000879771.1	TraY domain-containing protein	-
FIU12_RS00125	16441..16707	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FIU12_RS00130	16764..17005	-	242	Protein_24	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26723	26723
	-	flank	IS/Tn	-	-	13375..14319	944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.77 Da
• Isoelectric Point: 9.8619

>T127316 WP_001136729.1 NZ_CP040992:c12088-11774 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKTLPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDR
EMDRIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T127316 NZ_CP040992:c12088-11774 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGAGATTTACAACGCCTCCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGCCGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CATTTCTGCCGCTGGAATATCAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCGGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT127316 WP_000093040.1 NZ_CP040992:c12354-12076 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT127316 NZ_CP040992:c12354-12076 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AAGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCAGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: A0A060VNB1

Antitoxin

• AlphaFold DB: A0A2X1PRM1