Detailed information of TA system TA127315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2948..3422
• Replicon: plasmid unnamed1
• Accession: NZ_CP040992
• Organism: Klebsiella pneumoniae strain FDAARGOS_775

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: FIU12_RS00030
• Protein ID: WP_000262326.1
• Coordinates: 3156..3422 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: FIU12_RS00025
• Protein ID: WP_000879771.1
• Coordinates: 2948..3172 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU12_RS00010	90..1031	-	942	Protein_1	transposase	-
FIU12_RS00015	1018..1605	-	588	Protein_2	DEAD/DEAH box helicase	-
FIU12_RS00020	1633..2208	-	576	WP_000904911.1	recombinase family protein	-
FIU12_RS00025	2948..3172	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
FIU12_RS00030	3156..3422	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU12_RS00035	3479..3720	-	242	Protein_6	hypothetical protein	-
FIU12_RS00040	4047..5735	+	1689	WP_000012966.1	cloacin	-
FIU12_RS00045	5745..6002	+	258	WP_000523382.1	cloacin immunity family protein	-
FIU12_RS00050	6087..6236	+	150	WP_002193747.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26723	26723
	-	flank	IS/Tn	-	-	90..1034	944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T127315 WP_000262326.1 NZ_CP040992:3156-3422 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T127315 NZ_CP040992:3156-3422 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT127315 WP_000879771.1 NZ_CP040992:2948-3172 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT127315 NZ_CP040992:2948-3172 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACCTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCATCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5