Detailed information of TA system TA127311

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3209430..3210093
• Replicon: chromosome
• Accession: NZ_CP040933
• Organism: Edwardsiella ictaluri strain YCH

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: C5BAS3
• Locus tag: FGU63_RS15285
• Protein ID: WP_015872556.1
• Coordinates: 3209430..3209846 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C5BAS4
• Locus tag: FGU63_RS15290
• Protein ID: WP_015872557.1
• Coordinates: 3209827..3210093 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGU63_RS15265 (FGU63_15165)	3205354..3207087	-	1734	WP_015872552.1	single-stranded-DNA-specific exonuclease RecJ	-
FGU63_RS15270 (FGU63_15170)	3207092..3207808	-	717	WP_015872553.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
FGU63_RS15275 (FGU63_15175)	3207836..3208735	-	900	WP_015872554.1	site-specific tyrosine recombinase XerD	-
FGU63_RS15280 (FGU63_15180)	3208893..3209405	+	513	WP_015872555.1	flavodoxin FldB	-
FGU63_RS15285 (FGU63_15185)	3209430..3209846	-	417	WP_015872556.1	protein YgfX	Toxin
FGU63_RS15290 (FGU63_15190)	3209827..3210093	-	267	WP_015872557.1	FAD assembly factor SdhE	Antitoxin
FGU63_RS15295 (FGU63_15195)	3210441..3211436	+	996	WP_015872558.1	tRNA-modifying protein YgfZ	-
FGU63_RS15300 (FGU63_15200)	3211477..3212301	-	825	WP_015872559.1	shikimate 5-dehydrogenase	-
FGU63_RS15305 (FGU63_15205)	3212463..3213335	-	873	WP_015872560.1	nucleoside-specific channel-forming protein Tsx	-
FGU63_RS15310 (FGU63_15210)	3213944..3214593	+	650	Protein_2955	hemolysin III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16145.89 Da
• Isoelectric Point: 11.3233

>T127311 WP_015872556.1 NZ_CP040933:c3209846-3209430 [Edwardsiella ictaluri]
VALWRCNVHASWRTQVMFLLGHGALVLAILLAPWPENYSVFWLILLVAVIFECIRSQRLIARNSGELQRLAPQVWHWQLR
EWRLARRPWVSDLGALLILQSTHGTPCRRRLWLAADSMSREEWGQLRRALLDTGDDRV

Nucleotide

• Length: 417 bp

>T127311 NZ_CP040933:c3209846-3209430 [Edwardsiella ictaluri]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10364.92 Da
• Isoelectric Point: 5.6951

>AT127311 WP_015872557.1 NZ_CP040933:c3210093-3209827 [Edwardsiella ictaluri]
MDINNKARVHWACRRGMRELDIAIMPFFEHEYDALNDADKQTFVRLLSCDDPELFKWLMNQGKPDDTDLARMITLIQTRN
KERGPLAL

Nucleotide

• Length: 267 bp

>AT127311 NZ_CP040933:c3210093-3209827 [Edwardsiella ictaluri]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: C5BAS3

Antitoxin

• AlphaFold DB: C5BAS4