Detailed information of TA system TA127305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 895685..896348
• Replicon: chromosome
• Accession: NZ_CP040933
• Organism: Edwardsiella ictaluri strain YCH

Toxin (Protein)

• Gene name: tad
• Uniprot ID: C5B7U4
• Locus tag: FGU63_RS04250
• Protein ID: WP_015870280.1
• Coordinates: 896004..896348 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: FGU63_RS04245
• Protein ID: WP_015870279.1
• Coordinates: 895685..896002 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGU63_RS04200 (FGU63_04165)	890699..891115	+	417	WP_015870272.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
FGU63_RS04205 (FGU63_04170)	891212..891889	+	678	WP_015870273.1	envelope stress response activation lipoprotein NlpE	-
FGU63_RS04210 (FGU63_04175)	891911..892170	-	260	Protein_801	YfhL family 4Fe-4S dicluster ferredoxin	-
FGU63_RS04215 (FGU63_04185)	892775..893053	+	279	WP_133175613.1	hypothetical protein	-
FGU63_RS04225	893998..894135	+	138	WP_289245727.1	hypothetical protein	-
FGU63_RS04235	895150..895278	+	129	WP_015870277.1	hypothetical protein	-
FGU63_RS04240 (FGU63_04200)	895275..895652	+	378	WP_015870278.1	type II toxin-antitoxin system death-on-curing family toxin	-
FGU63_RS04245 (FGU63_04205)	895685..896002	-	318	WP_015870279.1	helix-turn-helix transcriptional regulator	Antitoxin
FGU63_RS04250 (FGU63_04210)	896004..896348	-	345	WP_015870280.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGU63_RS04255 (FGU63_04215)	896520..896738	-	219	WP_232347353.1	head completion/stabilization protein	-
FGU63_RS04260 (FGU63_04220)	896767..897456	+	690	WP_015870281.1	phage portal protein	-
FGU63_RS04265 (FGU63_04225)	898584..899431	-	848	Protein_812	MurR/RpiR family transcriptional regulator	-
FGU63_RS04270 (FGU63_04230)	899587..900231	+	645	WP_226092411.1	phosphatidylglycerophosphatase C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	895275..908342	13067
	-	flank	IS/Tn	-	-	894132..894740	608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12544.34 Da
• Isoelectric Point: 9.8474

>T127305 WP_015870280.1 NZ_CP040933:c896348-896004 [Edwardsiella ictaluri]
MKSLYWVGSTKKDLQSLPEEVQDIFGYALHLAQVGGKHSQTKPLKGFSGAGVLEVVEDFLGDTYRAVYTVKFGDAVYVSH
VFQKKSSSGIATPKLNMDKIRERLKAAENHARGA

Nucleotide

• Length: 345 bp

>T127305 NZ_CP040933:c896348-896004 [Edwardsiella ictaluri]
ATGAAGTCACTATATTGGGTTGGTAGTACAAAAAAAGACCTCCAGTCATTGCCTGAAGAAGTACAGGATATTTTCGGCTA
TGCCCTGCATCTGGCACAGGTAGGAGGTAAACACTCACAGACCAAGCCACTGAAGGGGTTTAGTGGAGCTGGCGTACTTG
AGGTAGTAGAAGACTTTCTCGGTGATACTTATCGTGCAGTGTATACCGTTAAGTTCGGTGATGCCGTCTACGTTTCGCAC
GTATTCCAGAAAAAGTCTTCATCGGGAATCGCCACGCCAAAGCTCAATATGGACAAGATCCGCGAACGGCTAAAAGCCGC
AGAAAACCATGCCAGAGGAGCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11480.41 Da
• Isoelectric Point: 10.2058

>AT127305 WP_015870279.1 NZ_CP040933:c896002-895685 [Edwardsiella ictaluri]
MSHDIEVSSGNVYVDIGRDDAEEMLLKAQLATTIGNIIKSRSLTQEQAAKLLGMTQPKLSNMLRGQFRGISEAKMLECLT
RLGRDVQIVVGKPRRTPGSVKVVLA

Nucleotide

• Length: 318 bp

>AT127305 NZ_CP040933:c896002-895685 [Edwardsiella ictaluri]
ATGAGCCATGATATTGAAGTCAGCAGCGGCAACGTTTATGTCGATATTGGCAGAGATGATGCCGAGGAAATGCTGTTAAA
AGCTCAGCTTGCCACCACTATTGGAAACATCATTAAAAGCCGTAGCCTGACGCAAGAACAAGCAGCAAAACTGCTGGGGA
TGACGCAGCCCAAACTGTCCAATATGCTGCGTGGGCAGTTCCGGGGCATCAGTGAAGCCAAAATGCTGGAATGTCTGACC
CGCTTAGGGCGCGACGTGCAAATCGTGGTTGGAAAGCCGCGCCGGACGCCGGGTAGCGTGAAAGTAGTGTTAGCCTGA

Structures

Toxin

• AlphaFold DB: C5B7U4