Detailed information of TA system TA127296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 101813..102611
• Replicon: plasmid pYY76-1-1
• Accession: NZ_CP040928
• Organism: Escherichia coli strain YY76-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: FH040_RS22900
• Protein ID: WP_077580870.1
• Coordinates: 102090..102611 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: FH040_RS22895
• Protein ID: WP_001711191.1
• Coordinates: 101813..102082 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH040_RS22870	97240..98580	-	1341	WP_023135655.1	AAA family ATPase	-
FH040_RS22875	98641..99366	-	726	WP_032187666.1	hypothetical protein	-
FH040_RS22880	99649..100416	+	768	WP_001711193.1	hypothetical protein	-
FH040_RS22885	100469..100822	-	354	WP_161953766.1	hypothetical protein	-
FH040_RS22890	100828..101493	-	666	WP_023135658.1	AAA family ATPase	-
FH040_RS22895	101813..102082	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
FH040_RS22900	102090..102611	+	522	WP_077580870.1	GNAT family N-acetyltransferase	Toxin
FH040_RS22910	102780..103031	-	252	WP_023135660.1	hypothetical protein	-
FH040_RS22915	103034..103726	-	693	WP_148245966.1	hypothetical protein	-
FH040_RS22920	103740..104063	-	324	WP_077580871.1	hypothetical protein	-
FH040_RS22925	104197..104778	-	582	WP_077580872.1	tail fiber assembly protein	-
FH040_RS22930	104787..105691	-	905	Protein_113	macro domain-containing protein	-
FH040_RS22935	105688..106644	-	957	Protein_114	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA12 / aadA2 / qacE / sul1 / mph(A) / lnu(F) / floR / aph(3')-Ia / tet(B)	-	1..163111	163111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19428.29 Da
• Isoelectric Point: 8.6369

>T127296 WP_077580870.1 NZ_CP040928:102090-102611 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKRLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T127296 NZ_CP040928:102090-102611 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAACGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT127296 WP_001711191.1 NZ_CP040928:101813-102082 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT127296 NZ_CP040928:101813-102082 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2