Detailed information of TA system TA127295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 95236..95834
• Replicon: plasmid pYY76-1-1
• Accession: NZ_CP040928
• Organism: Escherichia coli strain YY76-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: FH040_RS22855
• Protein ID: WP_053291281.1
• Coordinates: 95457..95834 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: FH040_RS22850
• Protein ID: WP_001603498.1
• Coordinates: 95236..95457 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH040_RS22830	90242..90790	+	549	WP_148245964.1	fimbrial protein YehD	-
FH040_RS22835	90846..91526	+	681	WP_148245968.1	fimbrial assembly chaperone	-
FH040_RS22840	91544..94030	+	2487	WP_148245965.1	fimbria/pilus outer membrane usher protein	-
FH040_RS22845	94041..95051	+	1011	WP_063078605.1	type 1 fimbrial protein	-
FH040_RS22850	95236..95457	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FH040_RS22855	95457..95834	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FH040_RS22865	95968..97083	-	1116	WP_032187668.1	DNA primase	-
FH040_RS22870	97240..98580	-	1341	WP_023135655.1	AAA family ATPase	-
FH040_RS22875	98641..99366	-	726	WP_032187666.1	hypothetical protein	-
FH040_RS22880	99649..100416	+	768	WP_001711193.1	hypothetical protein	-
FH040_RS22885	100469..100822	-	354	WP_161953766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA12 / aadA2 / qacE / sul1 / mph(A) / lnu(F) / floR / aph(3')-Ia / tet(B)	-	1..163111	163111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T127295 WP_053291281.1 NZ_CP040928:95457-95834 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T127295 NZ_CP040928:95457-95834 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT127295 WP_001603498.1 NZ_CP040928:95236-95457 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT127295 NZ_CP040928:95236-95457 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0