Detailed information of TA system TA127290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2445033..2445671
• Replicon: chromosome
• Accession: NZ_CP040927
• Organism: Escherichia coli strain YY76-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FH040_RS11940
• Protein ID: WP_000813794.1
• Coordinates: 2445495..2445671 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FH040_RS11935
• Protein ID: WP_001270286.1
• Coordinates: 2445033..2445449 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH040_RS11915	2440185..2441126	-	942	WP_001251313.1	ABC transporter permease	-
FH040_RS11920	2441127..2442140	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
FH040_RS11925	2442158..2443303	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FH040_RS11930	2443548..2444954	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
FH040_RS11935	2445033..2445449	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FH040_RS11940	2445495..2445671	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FH040_RS11945	2445893..2446123	+	231	WP_000494244.1	YncJ family protein	-
FH040_RS11950	2446215..2448176	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FH040_RS11955	2448249..2448785	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
FH040_RS11960	2448838..2450052	+	1215	WP_113402181.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2450092..2451357	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T127290 WP_000813794.1 NZ_CP040927:c2445671-2445495 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T127290 NZ_CP040927:c2445671-2445495 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT127290 WP_001270286.1 NZ_CP040927:c2445449-2445033 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT127290 NZ_CP040927:c2445449-2445033 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB