Detailed information of TA system TA127276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 760686..761284
• Replicon: chromosome
• Accession: NZ_CP040927
• Organism: Escherichia coli strain YY76-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: FH040_RS03685
• Protein ID: WP_053291281.1
• Coordinates: 760907..761284 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: FH040_RS03680
• Protein ID: WP_001603498.1
• Coordinates: 760686..760907 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH040_RS03665	756286..756966	+	681	WP_053270475.1	fimbrial assembly chaperone	-
FH040_RS03670	756984..759470	+	2487	WP_148245867.1	fimbria/pilus outer membrane usher protein	-
FH040_RS03675	759482..760501	+	1020	WP_095784823.1	type 1 fimbrial protein	-
FH040_RS03680	760686..760907	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FH040_RS03685	760907..761284	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FH040_RS03695	761394..761609	-	216	Protein_724	transposase	-
FH040_RS03700	761795..763057	-	1263	WP_113402210.1	integrase arm-type DNA-binding domain-containing protein	-
FH040_RS03710	763436..763669	-	234	Protein_726	DUF554 family protein	-
FH040_RS03715	763726..764423	+	698	Protein_727	IS1 family transposase	-
FH040_RS03720	764441..764920	-	480	Protein_728	DUF554 family protein	-
FH040_RS23840	765073..765156	+	84	WP_211180506.1	protein YqgH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	744985..764423	19438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T127276 WP_053291281.1 NZ_CP040927:760907-761284 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T127276 NZ_CP040927:760907-761284 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT127276 WP_001603498.1 NZ_CP040927:760686-760907 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT127276 NZ_CP040927:760686-760907 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0