Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 12394..12941 Replicon plasmid p853EC4
Accession NZ_CP040923
Organism Escherichia coli strain FC853_EC

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag FH485_RS27570 Protein ID WP_000969996.1
Coordinates 12394..12675 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID F4V9S6
Locus tag FH485_RS27575 Protein ID WP_000079931.1
Coordinates 12672..12941 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH485_RS27535 7665..8078 - 414 WP_106490004.1 hypothetical protein -
FH485_RS27540 8648..8905 + 258 WP_000343099.1 hypothetical protein -
FH485_RS27545 8905..9495 + 591 WP_106490005.1 hypothetical protein -
FH485_RS27555 10096..10689 + 594 WP_032274973.1 proQ/FINO family protein -
FH485_RS27560 10971..11558 - 588 WP_106490006.1 serine acetyltransferase -
FH485_RS27565 11858..12199 + 342 WP_000578011.1 hypothetical protein -
FH485_RS27570 12394..12675 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FH485_RS27575 12672..12941 - 270 WP_000079931.1 hypothetical protein Antitoxin
FH485_RS28040 13014..13154 - 141 WP_012512742.1 hypothetical protein -
FH485_RS27585 13851..14882 - 1032 WP_106490007.1 replication initiation protein -
FH485_RS27600 15854..16117 + 264 WP_032180564.1 ash family protein -
FH485_RS27605 16035..16322 + 288 WP_000074855.1 conjugal transfer protein TraA -
FH485_RS27615 16764..17069 + 306 Protein_23 transcription termination factor NusG -
FH485_RS27620 17083..17779 - 697 Protein_24 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCMY-42 - 1..45224 45224
- inside IScluster/Tn blaCMY-42 - 17504..24273 6769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T127269 WP_000969996.1 NZ_CP040923:c12675-12394 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T127269 NZ_CP040923:c12675-12394 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10194.55 Da        Isoelectric Point: 8.8735

>AT127269 WP_000079931.1 NZ_CP040923:c12941-12672 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT127269 NZ_CP040923:c12941-12672 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DLR5

References