127268

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 135773..136298 Replicon plasmid p853EC3
Accession NZ_CP040922
Organism Escherichia coli strain FC853_EC

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FH485_RS27420 Protein ID WP_001159868.1
Coordinates 135993..136298 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FH485_RS27415 Protein ID WP_000813634.1
Coordinates 135773..135991 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH485_RS27385 131166..131582 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
FH485_RS27390 131579..131809 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FH485_RS27395 132074..132574 + 501 WP_000528931.1 hypothetical protein -
FH485_RS27400 132587..133360 + 774 WP_000905949.1 hypothetical protein -
FH485_RS27405 133527..134660 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
FH485_RS27410 134694..135206 - 513 WP_000151784.1 hypothetical protein -
FH485_RS27415 135773..135991 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FH485_RS27420 135993..136298 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FH485_RS27425 136299..137105 + 807 WP_000016982.1 site-specific integrase -
FH485_RS27430 137879..138634 + 756 WP_024946706.1 replication initiation protein RepE -
FH485_RS27435 139222..140388 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / mph(A) / erm(B) / tet(B) / sitABCD iutA / iucD / iucC / iucB / iucA 1..143714 143714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T127268 WP_001159868.1 NZ_CP040922:135993-136298 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127268 NZ_CP040922:135993-136298 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT127268 WP_000813634.1 NZ_CP040922:135773-135991 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127268 NZ_CP040922:135773-135991 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References