Detailed information of TA system TA127266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 98825..99384
• Replicon: plasmid p853EC3
• Accession: NZ_CP040922
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: FH485_RS27205
• Protein ID: WP_000421272.1
• Coordinates: 99109..99384 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: FH485_RS27200
• Protein ID: WP_001387467.1
• Coordinates: 98825..99109 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS27170	94289..94729	-	441	Protein_108	zinc-binding dehydrogenase	-
FH485_RS27175	94703..94897	+	195	WP_000796505.1	hypothetical protein	-
FH485_RS27180	95130..96019	+	890	Protein_110	dihydrodipicolinate synthase family protein	-
FH485_RS27185	96044..96424	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
FH485_RS27190	96457..97422	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
FH485_RS27195	97468..98220	-	753	WP_000562172.1	IclR family transcriptional regulator	-
FH485_RS27200	98825..99109	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
FH485_RS27205	99109..99384	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FH485_RS27210	99490..99783	+	294	WP_001105060.1	hypothetical protein	-
FH485_RS27215	99818..99946	-	129	Protein_117	IS481 family transposase	-
FH485_RS27220	99979..101148	-	1170	WP_001072355.1	hypothetical protein	-
FH485_RS27230	101641..101948	+	308	Protein_119	type II toxin-antitoxin system ParD family antitoxin	-
FH485_RS27240	102291..102473	+	183	WP_042344623.1	hypothetical protein	-
FH485_RS27245	102594..103334	+	741	WP_001066953.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / mph(A) / erm(B) / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..143714	143714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T127266 WP_000421272.1 NZ_CP040922:99109-99384 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T127266 NZ_CP040922:99109-99384 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT127266 WP_001387467.1 NZ_CP040922:98825-99109 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT127266 NZ_CP040922:98825-99109 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880